Nature Communications (Jul 2020)
A protein-independent fluorescent RNA aptamer reporter system for plant genetic engineering
- Jiuyuan Bai,
- Yao Luo,
- Xin Wang,
- Shi Li,
- Mei Luo,
- Meng Yin,
- Yuanli Zuo,
- Guolin Li,
- Junyu Yao,
- Hua Yang,
- Mingdi Zhang,
- Wei Wei,
- Maolin Wang,
- Rui Wang,
- Chunhai Fan,
- Yun Zhao
Affiliations
- Jiuyuan Bai
- Key Laboratory of Bio-Resource and Eco-Environment of Ministry of Education, College of Life Sciences, Sichuan University
- Yao Luo
- State Key Laboratory and Collaborative Innovation Center of Biotherapy, West China Hospital, Sichuan University
- Xin Wang
- Key Laboratory of Bio-Resource and Eco-Environment of Ministry of Education, College of Life Sciences, Sichuan University
- Shi Li
- Key Laboratory of Bio-Resource and Eco-Environment of Ministry of Education, College of Life Sciences, Sichuan University
- Mei Luo
- Key Laboratory of Bio-Resource and Eco-Environment of Ministry of Education, College of Life Sciences, Sichuan University
- Meng Yin
- Key Laboratory of Bio-Resource and Eco-Environment of Ministry of Education, College of Life Sciences, Sichuan University
- Yuanli Zuo
- Key Laboratory of Bio-Resource and Eco-Environment of Ministry of Education, College of Life Sciences, Sichuan University
- Guolin Li
- Key Laboratory of Bio-Resource and Eco-Environment of Ministry of Education, College of Life Sciences, Sichuan University
- Junyu Yao
- Key Laboratory of Bio-Resource and Eco-Environment of Ministry of Education, College of Life Sciences, Sichuan University
- Hua Yang
- Key Laboratory of Bio-Resource and Eco-Environment of Ministry of Education, College of Life Sciences, Sichuan University
- Mingdi Zhang
- Key Laboratory of Bio-Resource and Eco-Environment of Ministry of Education, College of Life Sciences, Sichuan University
- Wei Wei
- Key Laboratory of Bio-Resource and Eco-Environment of Ministry of Education, College of Life Sciences, Sichuan University
- Maolin Wang
- Key Laboratory of Bio-Resource and Eco-Environment of Ministry of Education, College of Life Sciences, Sichuan University
- Rui Wang
- Key Laboratory of Bio-Resource and Eco-Environment of Ministry of Education, College of Life Sciences, Sichuan University
- Chunhai Fan
- School of Chemistry and Chemical Engineering, Frontiers Science Center for Transformative Molecules, Institute of Translational Medicine, Shanghai Jiao Tong University
- Yun Zhao
- Key Laboratory of Bio-Resource and Eco-Environment of Ministry of Education, College of Life Sciences, Sichuan University
- DOI
- https://doi.org/10.1038/s41467-020-17497-7
- Journal volume & issue
-
Vol. 11,
no. 1
pp. 1 – 14
Abstract
Fluorescent RNA aptamers could potentially be used as protein-independent reporters of transgene expression in plants. Here, the authors report that an optimized RNA aptamer, developed from Broccoli, can be used to detect transgene expression in stable and transiently transformed plant tissue.
