Characterization of d-xylose reductase, XyrB, from Aspergillus niger

Agata Terebieniec; Tania Chroumpi; Adiphol Dilokpimol; Maria Victoria Aguilar-Pontes; Miia R. Mäkelä; Ronald P. de Vries

Biotechnology Reports (Jun 2021)

Characterization of d-xylose reductase, XyrB, from Aspergillus niger

Agata Terebieniec,
Tania Chroumpi,
Adiphol Dilokpimol,
Maria Victoria Aguilar-Pontes,
Miia R. Mäkelä,
Ronald P. de Vries

Affiliations

Agata Terebieniec: Fungal Physiology, Westerdijk Fungal Biodiversity Institute and Fungal Molecular Physiology, Utrecht University, Utrecht, the Netherlands; Department of Molecular Biotechnology and Microbiology, Faculty of Chemistry, Gdansk University of Technology, Gdansk, Poland
Tania Chroumpi: Fungal Physiology, Westerdijk Fungal Biodiversity Institute and Fungal Molecular Physiology, Utrecht University, Utrecht, the Netherlands
Adiphol Dilokpimol: Fungal Physiology, Westerdijk Fungal Biodiversity Institute and Fungal Molecular Physiology, Utrecht University, Utrecht, the Netherlands
Maria Victoria Aguilar-Pontes: Fungal Physiology, Westerdijk Fungal Biodiversity Institute and Fungal Molecular Physiology, Utrecht University, Utrecht, the Netherlands
Miia R. Mäkelä: Department of Microbiology, University of Helsinki, Helsinki, Finland
Ronald P. de Vries: Fungal Physiology, Westerdijk Fungal Biodiversity Institute and Fungal Molecular Physiology, Utrecht University, Utrecht, the Netherlands; Corresponding author at: Westerdijk Fungal Biodiversity Institute, Uppsalalaan 8, 3584CT, Utrecht, the Netherlands.

Journal volume & issue: Vol. 30
p. e00610

Abstract

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d-xylose reductase is a member of the aldo-keto reductase family, and is involved in d-xylose and l-arabinose conversion through the Pentose Catabolic Pathway (PCP) in fungi. In this study, we biochemically characterized a newly identified second d-xylose reductase (XyrB) from Aspergillus niger. This NADPH-dependent reductase is able to efficiently convert d-xylose and l-arabinose, and it has the highest affinity for these sugars of all currently known fungal pentose reductases. A combination of biochemical data, transcriptomics and phylogenetic analysis further illustrated the role of XyrB in the PCP.Enzymes: D-xylose reductase (EC 1.1.1.307), L-arabinose reductase (EC 1.1.1.21).

Published in Biotechnology Reports

ISSN: 2215-017X (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Technology: Chemical technology: Biotechnology
Website: https://www.journals.elsevier.com/biotechnology-reports/

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