Protocol for inducing branching morphogenesis in human cholangiocyte and cholangiocarcinoma organoids

Kimberley Ober; Floris J.M. Roos; Gilles S. van Tienderen; Kübra Köten; Annelot Klaassen; Wunan Mi; Luc J.W. van der Laan; Monique M.A. Verstegen

STAR Protocols (Sep 2023)

Protocol for inducing branching morphogenesis in human cholangiocyte and cholangiocarcinoma organoids

Kimberley Ober,
Floris J.M. Roos,
Gilles S. van Tienderen,
Kübra Köten,
Annelot Klaassen,
Wunan Mi,
Luc J.W. van der Laan,
Monique M.A. Verstegen

Affiliations

Kimberley Ober: Department of Surgery, Erasmus MC Transplant Institute, University Medical Center Rotterdam, Rotterdam, the Netherlands; Corresponding author
Floris J.M. Roos: Wellcome-MRC Cambridge Stem Cell Institute, University of Cambridge, Cambridge, UK; Department of Surgery, University of Cambridge and NIHR Biomedical Research Centre, Cambridge, UK; Department of Surgery, Cambridge University Hospitals NHS Foundation Trust, Cambridge, UK
Gilles S. van Tienderen: Department of Surgery, Erasmus MC Transplant Institute, University Medical Center Rotterdam, Rotterdam, the Netherlands
Kübra Köten: Department of Surgery, Erasmus MC Transplant Institute, University Medical Center Rotterdam, Rotterdam, the Netherlands
Annelot Klaassen: Department of Surgery, Erasmus MC Transplant Institute, University Medical Center Rotterdam, Rotterdam, the Netherlands
Wunan Mi: Department of Surgery, Erasmus MC Transplant Institute, University Medical Center Rotterdam, Rotterdam, the Netherlands
Luc J.W. van der Laan: Department of Surgery, Erasmus MC Transplant Institute, University Medical Center Rotterdam, Rotterdam, the Netherlands
Monique M.A. Verstegen: Department of Surgery, Erasmus MC Transplant Institute, University Medical Center Rotterdam, Rotterdam, the Netherlands; Corresponding author

Journal volume & issue: Vol. 4, no. 3
p. 102431

Abstract

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Summary: Bile ducts are essential for bile transport and consist of complex branching tubular networks. Human patient-derived cholangiocyte develops a cystic rather than branching duct morphology. Here, we present a protocol to establish branching morphogenesis in cholangiocyte and cholangiocarcinoma organoids. We describe steps for the initiation, maintenance, and expansion of intrahepatic cholangiocyte organoids branching morphology. This protocol enables the study of organ-specific and mesenchymal-independent branching morphogenesis and provides an improved model to study biliary function and diseases.For complete details on the use and execution of this protocol, please refer to Roos et al. (2022).1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.

Published in STAR Protocols

ISSN: 2666-1667 (Online)
Publisher: Elsevier
Country of publisher: United States
LCC subjects: Science: Science (General)
Website: https://star-protocols.cell.com/

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