Kinesin-1 activity recorded in living cells with a precipitating dye

Simona Angerani; Eric Lindberg; Nikolai Klena; Christopher K. E. Bleck; Charlotte Aumeier; Nicolas Winssinger

doi:10.1038/s41467-021-21626-1

Nature Communications (Mar 2021)

Kinesin-1 activity recorded in living cells with a precipitating dye

Simona Angerani,
Eric Lindberg,
Nikolai Klena,
Christopher K. E. Bleck,
Charlotte Aumeier,
Nicolas Winssinger

Affiliations

Simona Angerani: School of Chemistry and Biochemistry, NCCR Chemical Biology, Faculty of Science, University of Geneva
Eric Lindberg: School of Chemistry and Biochemistry, NCCR Chemical Biology, Faculty of Science, University of Geneva
Nikolai Klena: Department of Cell Biology, Faculty of Science, University of Geneva
Christopher K. E. Bleck: Electron Microscopy Core Facility, National Heart, Lung and Blood Institute, National Institutes of Health
Charlotte Aumeier: School of Chemistry and Biochemistry, NCCR Chemical Biology, Faculty of Science, University of Geneva
Nicolas Winssinger: School of Chemistry and Biochemistry, NCCR Chemical Biology, Faculty of Science, University of Geneva

DOI: https://doi.org/10.1038/s41467-021-21626-1
Journal volume & issue: Vol. 12, no. 1
pp. 1 – 10

Abstract

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Monitoring the activity of the processive motor protein kinesin-1 in live cells is currently difficult. Here the authors report the fluorogenic small molecule QPD-OTf, a kinesin-1 substrate that causes activity-dependent dye precipitation.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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