Establishment of reference values based on influential characteristics of hematopoietic stem cells and immune cell subsets in the bone marrow
Rebar N. Mohammed,
Najmaddin S.H. Khoshnaw,
Vian Faeq Mohammed,
Dastan O. Hassan,
Chra Nawfal Abdullah,
Tavan Ismael Mahmood,
Huda A. Abbass,
Dereen Ahmed,
Kani D. Noori,
Lanja I. Saeed,
Salah Mohammed Salih,
Hiwa S. Sidiq,
Dlnya Omer Ali,
Alan Shwan,
Ignazio Majolino,
Francesco Ipsevich
Affiliations
Rebar N. Mohammed
Department of Medical Laboratory Technology, Faculty of Health Science, Qaiwan International University, Sulaimani, Kurdistan Region, Iraq; College of Veterinary Medicine, University of Sulaimani, Sulaymaniyah, Iraq; Bone Marrow Transplant Center, Hiwa Hospital, Sulaymaniyah, KRG, Iraq; Corresponding author. Bone marrow transplant centre, Hiwa Hospital, Sulaymaniyah, KRG, Iraq.
Najmaddin S.H. Khoshnaw
Department of Medical Laboratory Science, College of Science, Komar University of Science and Technology, KRG, Iraq; Department of Clinical Science, College of Medicine, University of Sulaimani, Sulaymaniyah, Iraq
Vian Faeq Mohammed
Bone Marrow Transplant Center, Hiwa Hospital, Sulaymaniyah, KRG, Iraq
Dastan O. Hassan
Bone Marrow Transplant Center, Hiwa Hospital, Sulaymaniyah, KRG, Iraq
Chra Nawfal Abdullah
Bone Marrow Transplant Center, Hiwa Hospital, Sulaymaniyah, KRG, Iraq
Tavan Ismael Mahmood
Bone Marrow Transplant Center, Hiwa Hospital, Sulaymaniyah, KRG, Iraq
Huda A. Abbass
Bone Marrow Transplant Center, Hiwa Hospital, Sulaymaniyah, KRG, Iraq
Dereen Ahmed
Bone Marrow Transplant Center, Hiwa Hospital, Sulaymaniyah, KRG, Iraq
Kani D. Noori
Bone Marrow Transplant Center, Hiwa Hospital, Sulaymaniyah, KRG, Iraq
Lanja I. Saeed
Bone Marrow Transplant Center, Hiwa Hospital, Sulaymaniyah, KRG, Iraq
Salah Mohammed Salih
Bone Marrow Transplant Center, Hiwa Hospital, Sulaymaniyah, KRG, Iraq
Hiwa S. Sidiq
Bone Marrow Transplant Center, Hiwa Hospital, Sulaymaniyah, KRG, Iraq
Dlnya Omer Ali
Bone Marrow Transplant Center, Hiwa Hospital, Sulaymaniyah, KRG, Iraq
Alan Shwan
Bone Marrow Transplant Center, Hiwa Hospital, Sulaymaniyah, KRG, Iraq
Ignazio Majolino
Ospedale San Camillo and Salvator Mundi International Hospital, Rome, Italy
Francesco Ipsevich
Ospedale San Camillo and Salvator Mundi International Hospital, Rome, Italy
Hematopoietic stem cell transplantation is still a curative treatment for many haematological cancers. Many factors, such as age, sex, ethnic background, smoking status, and body mass index, affect average reference values in different populations. This study aimed to establish a reference range for the absolute numbers and percentages of healthy individuals' hematopoietic stem cells and immune cells in the bone marrow. Seventy-one healthy donors (32 males and 39 females) were enrolled in the study. Following bone marrow harvesting, using flow cytometry, immunophenotyping was performed to determine the absolute number and percentage of CD34+ stem cells and various immune subsets. We found no statistically significant difference in the absolute count of HSCs or immune cell subsets in the bone marrow between males and females. Regarding age, the younger group had more significant CD34+ and immune cell subsets. Donors with healthier body weights tend to have richer bone marrow cellularity. Establishing a reference value for hematopoietic stem cells and immune cells in the bone marrow based on various influential factors is pivotal for defining bone marrow status and donor selection.
