Magnetic Nanoparticle-Based Electrochemical Sensing Platform Using Ferrocene-Labelled Peptide Nucleic Acid for the Early Diagnosis of Colorectal Cancer
Simge Balaban Hanoglu,
Ezgi Man,
Duygu Harmanci,
Serife Tozan Ruzgar,
Serdar Sanli,
Nazim Arda Keles,
Atakan Ayden,
Bilge Guvenc Tuna,
Ozgul Duzgun,
Omer Faruk Ozkan,
Soner Dogan,
Faezeh Ghorbanizamani,
Hichem Moulahoum,
Emine Guler Celik,
Serap Evran,
Suna Timur
Affiliations
Simge Balaban Hanoglu
Department of Biochemistry, Faculty of Science, Ege University, Bornova, Izmir 35100, Turkey
Ezgi Man
Department of Biochemistry, Faculty of Science, Ege University, Bornova, Izmir 35100, Turkey
Duygu Harmanci
Central Research Test and Analysis Laboratory, Application and Research Center, Ege University, Bornova, Izmir 35100, Turkey
Serife Tozan Ruzgar
Central Research Test and Analysis Laboratory, Application and Research Center, Ege University, Bornova, Izmir 35100, Turkey
Serdar Sanli
Department of Biochemistry, Faculty of Science, Ege University, Bornova, Izmir 35100, Turkey
Nazim Arda Keles
Department of Medical Biology, School of Medicine, Yeditepe University, Istanbul 34755, Turkey
Atakan Ayden
Department of Medical Biology, School of Medicine, Yeditepe University, Istanbul 34755, Turkey
Bilge Guvenc Tuna
Department of Biophysics, School of Medicine, Yeditepe University, Istanbul 34755, Turkey
Ozgul Duzgun
Department of Surgical Oncology, University of Health Sciences, Umraniye Training and Research Hospital, Istanbul 34764, Turkey
Omer Faruk Ozkan
Department of Surgical Oncology, University of Health Sciences, Umraniye Training and Research Hospital, Istanbul 34764, Turkey
Soner Dogan
Department of Medical Biology, School of Medicine, Yeditepe University, Istanbul 34755, Turkey
Faezeh Ghorbanizamani
Department of Biochemistry, Faculty of Science, Ege University, Bornova, Izmir 35100, Turkey
Hichem Moulahoum
Department of Biochemistry, Faculty of Science, Ege University, Bornova, Izmir 35100, Turkey
Emine Guler Celik
Department of Bioengineering, Faculty of Engineering, Ege University, Bornova, Izmir 35100, Turkey
Serap Evran
Department of Biochemistry, Faculty of Science, Ege University, Bornova, Izmir 35100, Turkey
Suna Timur
Department of Biochemistry, Faculty of Science, Ege University, Bornova, Izmir 35100, Turkey
Diagnostic biomarkers based on epigenetic changes such as DNA methylation are promising tools for early cancer diagnosis. However, there are significant difficulties in directly and specifically detecting methylated DNA regions. Here, we report an electrochemical sensing system based on magnetic nanoparticles that enable a quantitative and selective analysis of the methylated septin9 (mSEPT9) gene, which is considered a diagnostic marker in early stage colorectal cancer (CRC). Methylation levels of SEPT9 in CRC samples were successfully followed by the selective recognition ability of a related peptide nucleic acid (PNA) after hybridization with DNA fragments in human patients’ serums and plasma (n = 10). Moreover, this system was also adapted into a point-of-care (POC) device for a one-step detection platform. The detection of mSEPT9 demonstrated a limit of detection (LOD) value of 0.37% and interference-free measurement in the presence of branched-chain amino acid transaminase 1 (BCAT1) and SRY box transcription factor 21 antisense divergent transcript 1 (SOX21-AS1). The currently proposed functional platform has substantial prospects in translational applications of early CRC detection.
