Monochromic radiation through light-emitting diode (LED) positively augments in vitro shoot regeneration in Orchid (Dendrobium sonia)

Abstract

Monochromatic lights emitted by light-emitting diodes (LEDs) have generated great interest for efficient and controlled growth in vitro, especially of plants which are endangered or require specific intensity and wavelength of light. In the present study, we have evaluated the effect of monochromatic LEDs on in vitro morphogenesis: growth, proliferation of shoot cultures, and rooting of Dendrobium sonia. Different light sources viz. white LEDs (W), blue LEDs (B), yellow LEDs (Y) and red LEDs (R) were tested under photoperiod of 16 h of exposure and 8 h of dark. The frequency of morphogenesis depended on the wavelength of the applied monochromatic light. Higher wavelength monochromatic light (yellow light) was observed to induce higher shoot proliferation (98%), early PLB (protocorm-like bodies) formation, differentiation into green buds and shoot initiation as compared to red, blue and white light treatments. Yellow light also yielded higher number of shoots per explants (29 shoots/explant) than red, blue and white light treatments. The results suggest that the monochromatic light sources stimulate morphogenic effects on in vitro culture of Dendrobium sonia, and that yellow light treatment can be used to enhance the efficiency of micropropagation.