Identification of aberrantly expressed long non‐coding RNAs in ovarian high‐grade serous carcinoma cells

Abstract

Abstract Purpose To identify the aberrantly expressed long non‐coding RNAs (lncRNAs) in ovarian high‐grade serous carcinoma (HGSC). Methods Total RNA was isolated in HGSC cell lines, ovarian surface epithelial cells, and normal ovaries. Aberrantly expressed lncRNAs in HGSC were identified by PCR array, which analyzes 84 kinds of lncRNAs. To infer their functions, HGSC cell lines with different levels of expression of the identified lncRNAs were established, and then, activities of proliferation, migration, and apoptosis were examined. Expression levels of the identified lncRNAs were also examined in multiple ovarian HGSC tissues. Results Ten aberrantly expressed lncRNAs, six upregulated and four downregulated, were identified in the HGSC cell lines. The authors established four HGSC cell lines: in two of the cell lines, one of the upregulated lncRNAs was knocked down, and in two other cell lines, one of the downregulated lncRNAs (MEG3 and POU5F1P5) was overexpressed. Migration activities were inhibited in the HGSC cell lines overexpressing MEG3 or POU5F1P5 while there were no differences in proliferation and apoptosis between the established and control cell lines. The four lncRNAs downregulated in the HGSC cell lines were also observed to be downregulated in ovarian HGSC tissues. Conclusion The authors identified four downregulated lncRNAs in ovarian HGSC.

Keywords

• long non‐coding RNAs (lncRNAs)
• MEG3
• ovarian cancer
• ovarian high‐grade serous carcinoma (HGSC)
• POU5F1P5