Nature Communications (Aug 2022)
SARS-CoV-2 variants of concern: spike protein mutational analysis and epitope for broad neutralization
- Dhiraj Mannar,
- James W. Saville,
- Zehua Sun,
- Xing Zhu,
- Michelle M. Marti,
- Shanti S. Srivastava,
- Alison M. Berezuk,
- Steven Zhou,
- Katharine S. Tuttle,
- Michele D. Sobolewski,
- Andrew Kim,
- Benjamin R. Treat,
- Priscila Mayrelle Da Silva Castanha,
- Jana L. Jacobs,
- Simon M. Barratt-Boyes,
- John W. Mellors,
- Dimiter S. Dimitrov,
- Wei Li,
- Sriram Subramaniam
Affiliations
- Dhiraj Mannar
- Department of Biochemistry and Molecular Biology, University of British Columbia
- James W. Saville
- Department of Biochemistry and Molecular Biology, University of British Columbia
- Zehua Sun
- Center for Antibody Therapeutics, Division of Infectious Diseases, Department of Medicine, University of Pittsburgh School of Medicine
- Xing Zhu
- Department of Biochemistry and Molecular Biology, University of British Columbia
- Michelle M. Marti
- Department of Infectious Diseases and Microbiology, Graduate School of Public Health, University of Pittsburgh
- Shanti S. Srivastava
- Department of Biochemistry and Molecular Biology, University of British Columbia
- Alison M. Berezuk
- Department of Biochemistry and Molecular Biology, University of British Columbia
- Steven Zhou
- Department of Biochemistry and Molecular Biology, University of British Columbia
- Katharine S. Tuttle
- Department of Biochemistry and Molecular Biology, University of British Columbia
- Michele D. Sobolewski
- Division of Infectious Diseases, Department of Medicine, University of Pittsburgh School of Medicine
- Andrew Kim
- Center for Antibody Therapeutics, Division of Infectious Diseases, Department of Medicine, University of Pittsburgh School of Medicine
- Benjamin R. Treat
- Department of Infectious Diseases and Microbiology, Graduate School of Public Health, University of Pittsburgh
- Priscila Mayrelle Da Silva Castanha
- Department of Infectious Diseases and Microbiology, Graduate School of Public Health, University of Pittsburgh
- Jana L. Jacobs
- Division of Infectious Diseases, Department of Medicine, University of Pittsburgh School of Medicine
- Simon M. Barratt-Boyes
- Department of Infectious Diseases and Microbiology, Graduate School of Public Health, University of Pittsburgh
- John W. Mellors
- Division of Infectious Diseases, Department of Medicine, University of Pittsburgh School of Medicine
- Dimiter S. Dimitrov
- Center for Antibody Therapeutics, Division of Infectious Diseases, Department of Medicine, University of Pittsburgh School of Medicine
- Wei Li
- Center for Antibody Therapeutics, Division of Infectious Diseases, Department of Medicine, University of Pittsburgh School of Medicine
- Sriram Subramaniam
- Department of Biochemistry and Molecular Biology, University of British Columbia
- DOI
- https://doi.org/10.1038/s41467-022-32262-8
- Journal volume & issue
-
Vol. 13,
no. 1
pp. 1 – 12
Abstract
SARS-CoV-2 variants have accumulated multiple defining mutations within their spike glycoproteins. Here, the authors report a structural basis for broad neutralization of several variants by a heavy chain antibody fragment and provide a mutational analysis focusing on antibody evasion, receptor engagement, and spike protein structure.
