Novel immuno-nucleic acid cooperative detection technology for food safety

Abstract

The establishment of nucleic acid amplification technology and functional nucleic acid aptamers promote the development of detection techniques. In particular, the detection displayed promise for broader application, as well as improved specificity ad higher sensitivity when combined with immunoassays (IA). This review introduced the novel immuno-nucleic acid (INA) cooperative detection technology, which is currently applied in the field of food safety for the recognition of residues of veterinary drugs and pesticides, biological toxins, pathogenic microorganisms, genetically modified foods (GMF), environmental pollutants, and more. Ultimately, the prospects for future development prospects, as well as the potential advantages of this technology were presented. Graphic. Magnifying glass, Taiji, Circular ring, Octagonal flowers, and Seven circular illustrations. Magnifying glass represents immunoassay (IA). Nucleic acid amplification and functional nucleic acid aptamers represent the yellow part and the green part of Taiji, respectively. The light blue circular ring is written “more sensitive, more specific, more rapid, low cost, high throughput” shows advantages of immunoassays combined nucleic acid amplification and functional nucleic acid aptamers. Each extended petals of octagonal flowers represent the application of immuno-nucleic acid cooperative detection technology for food safety in pesticide residues, veterinary drug residues, toxin, pathogenic microorganisms, genetically modified food, food additive, as well as allergen and harmful substance, respectively. Finally, the seven circular illustrations display the most representative elements in seven detection fields above intuitively.

Keywords

• food safety
• immunoassay
• nucleic acid amplification technology
• functional nucleic acid
• aptamers
• immuno-nucleic acid cooperative detection