Formulation, Characterization and Permeability Studies of Fenugreek (<i>Trigonella foenum-graecum</i>) Containing Self-Emulsifying Drug Delivery System (SEDDS)
Dávid Sinka,
Enikő Doma,
Nóra Szendi,
Jázmin Páll,
Dóra Kósa,
Ágota Pető,
Pálma Fehér,
Zoltán Ujhelyi,
Ferenc Fenyvesi,
Judit Váradi,
Miklós Vecsernyés,
Zsolt Szűcs,
Sándor Gonda,
Zoltán Cziáky,
Attila Kiss-Szikszai,
Gábor Vasas,
Ildikó Bácskay
Affiliations
Dávid Sinka
Department of Pharmaceutical Technology, Faculty of Pharmacy, University of Debrecen, Nagyerdei Körút 98, 4032 Debrecen, Hungary
Enikő Doma
Department of Pharmaceutical Technology, Faculty of Pharmacy, University of Debrecen, Nagyerdei Körút 98, 4032 Debrecen, Hungary
Nóra Szendi
Department of Pharmaceutical Technology, Faculty of Pharmacy, University of Debrecen, Nagyerdei Körút 98, 4032 Debrecen, Hungary
Jázmin Páll
Department of Pharmaceutical Technology, Faculty of Pharmacy, University of Debrecen, Nagyerdei Körút 98, 4032 Debrecen, Hungary
Dóra Kósa
Department of Pharmaceutical Technology, Faculty of Pharmacy, University of Debrecen, Nagyerdei Körút 98, 4032 Debrecen, Hungary
Ágota Pető
Department of Pharmaceutical Technology, Faculty of Pharmacy, University of Debrecen, Nagyerdei Körút 98, 4032 Debrecen, Hungary
Pálma Fehér
Department of Pharmaceutical Technology, Faculty of Pharmacy, University of Debrecen, Nagyerdei Körút 98, 4032 Debrecen, Hungary
Zoltán Ujhelyi
Department of Pharmaceutical Technology, Faculty of Pharmacy, University of Debrecen, Nagyerdei Körút 98, 4032 Debrecen, Hungary
Ferenc Fenyvesi
Department of Pharmaceutical Technology, Faculty of Pharmacy, University of Debrecen, Nagyerdei Körút 98, 4032 Debrecen, Hungary
Judit Váradi
Department of Pharmaceutical Technology, Faculty of Pharmacy, University of Debrecen, Nagyerdei Körút 98, 4032 Debrecen, Hungary
Miklós Vecsernyés
Department of Pharmaceutical Technology, Faculty of Pharmacy, University of Debrecen, Nagyerdei Körút 98, 4032 Debrecen, Hungary
Zsolt Szűcs
Department of Pharmacognosy, University of Debrecen, Nagyerdei Körút 98, 4032 Debrecen, Hungary
Sándor Gonda
Department of Pharmacognosy, University of Debrecen, Nagyerdei Körút 98, 4032 Debrecen, Hungary
Zoltán Cziáky
Agricultural and Molecular Research and Service Institute, University of Nyíregyháza, Sóstói út 31/b, 4400 Nyíregyháza, Hungary
Attila Kiss-Szikszai
Department of Organic Chemistry, University of Debrecen, Egyetem tér 1, 4010 Debrecen, Hungary
Gábor Vasas
Department of Pharmacognosy, University of Debrecen, Nagyerdei Körút 98, 4032 Debrecen, Hungary
Ildikó Bácskay
Department of Pharmaceutical Technology, Faculty of Pharmacy, University of Debrecen, Nagyerdei Körút 98, 4032 Debrecen, Hungary
Fenugreek is used as a spice and a traditional herbal medicine for a variety of purposes, given its antidiabetic and antioxidant effects. Self-emulsifying drug delivery systems (SEDDS) of herbal drugs are targets of extensive research aiming to increase bioavailability and stability. The study’s objective was to formulate SEDDS containing Trigonella foenum-graecum extract to improve the stability of herbal extract and to increase their permeability through a Caco-2 monolayer. A characterized fenugreek dry extract was used for the formulations, while the SEDDS properties were examined by particle size analysis and zeta potential measurements. Permeability assays were carried out on Caco-2 cell monolayers, the integrity of which was monitored by follow-up trans-epithelial electric resistance measurements (TEER). Cytocompatibility was tested by the MTT method, and an indirect dissolution test was performed, using DPPH antioxidant reagent. Two different SEDDS compositions were formulated from a standardized fenugreek dry extract at either the micro- or the nanoemulsion scale with sufficient stability, enhanced bioavailability of the compounds, and sustained release from HPMC capsules. Based on our results, a modern, non-toxic, cytocompatible fenugreek SEDDS formulation with high antioxidant capacity was developed in order to improve the permeability and bioavailability of all components.