Relationship between Glutathione-Dependent Enzymes and the Immunohistochemical Profile of Glial Neoplasms
Larisa Obukhova,
Tatiana Kopytova,
Elena Murach,
Natalya Shchelchkova,
Claudia Kontorshchikova,
Igor Medyanik,
Natalia Orlinskaya,
Artem Grishin,
Michael Kontorshchikov,
Dariya Badanina
Affiliations
Larisa Obukhova
Federal State Budgetary Educational Institution of Higher Education, The “Privolzhsky Research Medical University” of the Ministry of Health of the Russian Federation, 603005 Nizhny Novgorod, Russia
Tatiana Kopytova
Federal State Budgetary Educational Institution of Higher Education, The “Privolzhsky Research Medical University” of the Ministry of Health of the Russian Federation, 603005 Nizhny Novgorod, Russia
Elena Murach
Federal State Budgetary Educational Institution of Higher Education, The “Privolzhsky Research Medical University” of the Ministry of Health of the Russian Federation, 603005 Nizhny Novgorod, Russia
Natalya Shchelchkova
Federal State Budgetary Educational Institution of Higher Education, The “Privolzhsky Research Medical University” of the Ministry of Health of the Russian Federation, 603005 Nizhny Novgorod, Russia
Claudia Kontorshchikova
Federal State Budgetary Educational Institution of Higher Education, The “Privolzhsky Research Medical University” of the Ministry of Health of the Russian Federation, 603005 Nizhny Novgorod, Russia
Igor Medyanik
Federal State Budgetary Educational Institution of Higher Education, The “Privolzhsky Research Medical University” of the Ministry of Health of the Russian Federation, 603005 Nizhny Novgorod, Russia
Natalia Orlinskaya
Federal State Budgetary Educational Institution of Higher Education, The “Privolzhsky Research Medical University” of the Ministry of Health of the Russian Federation, 603005 Nizhny Novgorod, Russia
Artem Grishin
Federal State Budgetary Educational Institution of Higher Education, The “Privolzhsky Research Medical University” of the Ministry of Health of the Russian Federation, 603005 Nizhny Novgorod, Russia
Michael Kontorshchikov
Federal State Budgetary Educational Institution of Higher Education, The “Privolzhsky Research Medical University” of the Ministry of Health of the Russian Federation, 603005 Nizhny Novgorod, Russia
Dariya Badanina
Federal State Budgetary Educational Institution of Higher Education, The “Privolzhsky Research Medical University” of the Ministry of Health of the Russian Federation, 603005 Nizhny Novgorod, Russia
This research aimed to investigate the relationships between the parameters of glutathione metabolism and the immunohistochemical characteristics of glial tumors. Postoperative material from 20 patients with gliomas of different grades of anaplasia was analyzed. Bioinformatic analysis of the interactions between the gliomas’ immunohistochemical markers and their glutathione-dependent enzymes was carried out using the STRING, BioGrid, while Signor databases revealed interactions between such glioma markers as IDH and p53 and the glutathione exchange enzymes (glutathione peroxidase, glutathione reductase, glutathione S-transferase). The most pronounced relationship with glutathione metabolism was demonstrated by the level of the nuclear protein Ki67 as a marker of proliferative activity, and the presence of the IDH1 mutation as one of the key genetic events of gliomagenesis. The glutathione system is an active participant in the body’s antioxidant defense, involving the p53 markers and MGMT promoter methylation. It allows characterization of the gliomal cells’ status at different stages of tumor development.
