Nature Communications (Sep 2019)

The ALFA-tag is a highly versatile tool for nanobody-based bioscience applications

  • Hansjörg Götzke,
  • Markus Kilisch,
  • Markel Martínez-Carranza,
  • Shama Sograte-Idrissi,
  • Abirami Rajavel,
  • Thomas Schlichthaerle,
  • Niklas Engels,
  • Ralf Jungmann,
  • Pål Stenmark,
  • Felipe Opazo,
  • Steffen Frey

DOI
https://doi.org/10.1038/s41467-019-12301-7
Journal volume & issue
Vol. 10, no. 1
pp. 1 – 12

Abstract

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Epitope tags are widely used in various applications, but often lack versatility. Here, the authors introduce a small, alpha helical tag, which is recognized by a high affinity nanobody and can be used in a range of different applications, from protein purification to super-resolution imaging and in vivo detection of proteins.