Nature Communications (Sep 2019)
The ALFA-tag is a highly versatile tool for nanobody-based bioscience applications
Abstract
Epitope tags are widely used in various applications, but often lack versatility. Here, the authors introduce a small, alpha helical tag, which is recognized by a high affinity nanobody and can be used in a range of different applications, from protein purification to super-resolution imaging and in vivo detection of proteins.