Optimization of Callogenesis and Regeneration of Cucumber (Cucumis Sativus L.) UtilizingCotyledon, Hypocotyl and Leaf Explants

Abstract

Supernia is one of the most important high-yield cultivars of the imported cucumber hybrids. In order to optimize asexual propagation of this variety through in vitro culture, cotyledon, hypocotyl and leaf explants were placed in the MS basal medium containing 0, 0.1, 0.2, 0.3 and 0.4 mg/l concentrations of 1-Naphthaleneacetic acid (NAA) in combination with 1, 2, 3 and 4 mg/l of 6-Benzylaminopurine (BAP) hormone. The experiment was conducted as a factorial arrangement in completely randomized design at three replications and five samples in each replicate. The percentage of callogenesis, embryogenic calli and shoot frequency of the cultured explants were evaluated. The highest percentage of callogenesis (86.6%) was obtained from cotyledon explant on the MS medium supplemented with 0.3 mg/l NAA + 3 mg/l BAP hormones. The highest embryogenic calli (61.6%) were developed on the MS medium supplemented with 0.2 mg/l NAA + 2 mg/l BAP hormones in hypocotyl culture. Also, the maximum number of shoot (20.08) were obtained on MS medium supplemented with 0.3 mg/l NAA + 3 mg/l BAP hormones in hypocotyl explant. The regenerated shoots were transferred to half-strength MS medium supplemented with 1 mg/l NAA hormone for root induction and formation. Then, the grown seedlings were adapted to the in vivo condition. Based on the results of this study, it is recommended to use the cotyledon explant for propagation and regeneration of this genotype, especially in the gene transferring studies.

Keywords

• micro propagation
• in vitro culture
• cucumber
• naa
• bap