Rapid detection of enrofloxacin and ciprofloxacin in milk by Raman spectroscopy based on detection probability model

Abstract

ObjectiveTo establish a rapid detection method for enrofloxacin and ciprofloxacin in milk products by surface enhanced Raman spectroscopy.MethodsFive percent acetic acid was used for extraction and dichloromthane was used for liquid-liquid distribution and purification. The parameters including addition amount of colloidal silver， extraction solution and coagulant were determined by single factor experiment.ResultsRaman characteristic shifts of enrofloxacin and ciprofloxacin were （736±5）cm-1， （785±5）cm-1， （1 345±5）cm-1 and （1 395±5）cm-1. According to the position and intensity of probability of detection model， the detection limit of enrofloxacin and ciprofloxacin in milk was 50 μg/kg.ConclusionThe method is simple in pretreatment， the detection is fast and the result is accurate. It only takes 25 min from sample pretreatment to the result issuing. It can be used for the rapid on-site qualitative determination of enrofloxacin and ciprofloxacin in milk.

Keywords

• enrofloxacin
• ciprofloxacin
• surface enhanced raman spectroscopy
• probability of detection
• field rapid detection