Data on Manuka Honey/Gellan Gum composite hydrogels for cartilage repair
Maria A. Bonifacio,
Stefania Cometa,
Andrea Cochis,
Piergiorgio Gentile,
Ana M. Ferreira,
Barbara Azzimonti,
Giuseppe Procino,
Edmondo Ceci,
Lia Rimondini,
Elvira De Giglio
Affiliations
Maria A. Bonifacio
Department of Chemistry, University of Bari “Aldo Moro”, via Orabona 4, 70126 Bari, Italy
Stefania Cometa
Jaber Innovation s.r.l., via Calcutta 8, 00144 Rome, Italy
Andrea Cochis
Department of Health Sciences, University of Piemonte Orientale “UPO”, via Solaroli 17, 28100 Novara, Italy
Piergiorgio Gentile
School of Engineering, Newcastle University, NE1 7RU Newcastle upon Tyne, UK
Ana M. Ferreira
School of Engineering, Newcastle University, NE1 7RU Newcastle upon Tyne, UK
Barbara Azzimonti
Department of Health Sciences, University of Piemonte Orientale “UPO”, via Solaroli 17, 28100 Novara, Italy; INSTM, Consorzio Interuniversitario Nazionale per la Scienza e Tecnologia dei Materiali, via Giuseppe Giusti 9, 50121 Firenze, Italy
Giuseppe Procino
Department of Biosciences, Biotechnologies and Biopharmaceutics, University of Bari “Aldo Moro”, via Orabona 4, 70126 Bari, Italy
Edmondo Ceci
Department of Veterinary Medicine, University of Bari “Aldo Moro”, Str. Prov. Casamassima Km 3, 70010 Valenzano, Italy
Lia Rimondini
Department of Health Sciences, University of Piemonte Orientale “UPO”, via Solaroli 17, 28100 Novara, Italy; INSTM, Consorzio Interuniversitario Nazionale per la Scienza e Tecnologia dei Materiali, via Giuseppe Giusti 9, 50121 Firenze, Italy
Elvira De Giglio
Department of Chemistry, University of Bari “Aldo Moro”, via Orabona 4, 70126 Bari, Italy; Corresponding author.
This work contains original data supporting our research paper “Antibacterial effectiveness meets improved mechanical properties: Manuka Honey/Gellan Gum composite hydrogels for cartilage repair”, Bonifacio et al., in press [1], in which innovative composite hydrogels, based on Gellan Gum/Manuka honey/Halloysite nanotubes were described as biomaterials for cartilage regeneration. Here the composites were further examined by means of Fourier Transform Infrared Spectroscopy, in Attenuated Total Reflectance mode (FT-IR/ATR). Materials devoted to cartilage replacement must possess adequate fluid permeability and lubricating capability, therefore, a deeper investigation on water uptake kinetics of freeze-dried specimens up to 21 days in PBS was carried out. Moreover, since the degradation rate of a biomaterial plays a pivotal role in tissue engineering, weight loss measurements of the prepared hydrogels were performed in simulated synovial fluid, in phosphate buffer solution (PBS) and in lysozyme. Scanning Electron Microscopy images provide insight into the morphology of the freeze-dried samples.Finally, additional information on Staphylococcus aureus and Staphylococcus epidermidis ability to adhere onto the prepared hydrogel composites in short times were obtained, as well as the chondrogenic potential of the composites assessed by SDS-PAGE followed by Coomassie blue gel staining.
