Investigation of Possible Genotoxic and Cytotoxic Effects of Differential Boron Compounds in CCL 62 (Hela Contaminant) Human Amniotic Ephitelial Cell Line

Abstract

Epidemiological and in vitro studies have showed that boron may have anti-carcinogenic properties. Chromosome aberrations assay and sister chromatid exchange assays have showed that boron has no genotoxic effects on cytogenetically stable cell lines. Aim of this investigation is determine to effects of different boron compounds which are boric acid (BA), borax pentahydrate (BP) and disodium pentaborate decahydrate (DPD) ) on exist cytogenetic disruption with variable doses (250, 500 and 1000µM ) on cytogenitically unstabel CCL 62 (HeLa Contaminant) cell line. In order to test this hypothesis, following the boron treatment on the cell lines for cytotoxcity was performed using MTT that cell viability assay. For genotoxicity was used chromosome aberrations assay (CAs) and micronucleus assay (MN), CAs and MN frequency calculated in each boron dose. Boron compaunds effected proliferation of CCL 62 (human amniotic ephitelial) cell lines in a time, species and dose dependent manner. According to data obtained from CAs and MN assays, no significant difference was found between control groups which were not treated any of boron compaund with boron treated groups (p>0,05). In conclusion, we established that BA, BP, and DPD effected proliferation of CCL 62 cell lines in a time, compaund and dose dependent manner, however no evidence was observed suggesting these compounds cause an increase or decrease in the level of existing cytogenetic defects in these cell lines. [Med-Science 2013; 2(1.000): 454-68]

Keywords

• Boron
• Boric acid
• Borax pentahydrate
• Disodium pentaborate decahydrate
• Cytotoxicity
• Genotoxicity