精准医学杂志 (Apr 2024)

Effect of moderate fatty liver on cardiac function in orthotopic autologous liver transplantation model rats

  • WANG Kaiwen, DONG He, LYU Lin, LIU Huan, CAI Jinzhen

DOI
https://doi.org/10.13362/j.jpmed.202402009
Journal volume & issue
Vol. 39, no. 2
pp. 139 – 143

Abstract

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Objective To explore the effect of moderate fatty liver on cardiac function in orthotopic autologous liver transplantation model rats. Methods Sixty SPF-grade healthy male SD rats were randomly divided into three groups (20 rats in each group). Group A was fed ordinary diet for 6 weeks and underwent laparotomy followed by separation of the blood vessels and ligaments around the liver and abdominal closure; group B was fed ordinary diet for 6 weeks and underwent orthotopic autologous liver transplantation; group C was fed high-fat diet for 6 weeks and underwent orthotopic autologous liver transplantation. The body mass, liver wet mass, liver index, and serum levels of aspartate aminotransferase (AST) and alanine aminotransferase (ALT) after 6 weeks of feeding, as well as intraoperative blood pressure, pulse rate, respiratory rate, proportion of arrhythmia occurring in the new liver stage, and serum levels of lactate dehydrogenase (LDH), α-hydroxybutyrate dehydrogenase (α-HBDH), creatine kinase (CK), creatine kinase isoenzyme (CK-MB), and high-sensitivity cardiac troponin (hs-cTn) at 0, 5, 10, and 60 min in the new liver stage were compared. Results The body mass, liver wet mass, liver index, and serum AST and ALT levels, as well as intraoperative systolic blood pressure, diastolic blood pressure, pulse rate, respiratory rate, and proportion of arrhythmia occurring in the new liver stage in group C were significantly higher than those in groups A and B (F=36.40-4 060.54,χ2 =10.91,P<0.05), with no significant differences between groups A and B (P>0.05). The serum levels of LDH, α-HBDH, CK, CK-MB, and hs-cTn at different time points in the new liver stage were significantly higher in group C than in groups A and B (F=14.11-1 057.08,P<0.05), and were significantly higher in group B than in group A (F=12.59-279.94,P<0.05). Conclusion Moderate fatty liver may lead to the occurrence of arrhythmia in the new liver stage in liver transplantation, reduce cardiac function, and exacerbate intraoperative stress response.

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