Genome-Wide Association Study on Seedling Phenotypic Traits of Wheat under Different Nitrogen Conditions
Chenchen Hu,
Jinghui Li,
Jiajia Liu,
Dazhong Zhang,
Liqiao Jin,
Nian Yang,
Bipo Bai,
Zenghao Wang,
Suwei Feng,
Zhengang Ru,
Tiezhu Hu
Affiliations
Chenchen Hu
Henan Provincial Key Laboratory of Hybrid Wheat, Henan Institute of Science and Technology, Collaborative Innovation Center of Modern Biological Breeding, Xinxiang 453003, China
Jinghui Li
Henan Provincial Key Laboratory of Hybrid Wheat, Henan Institute of Science and Technology, Collaborative Innovation Center of Modern Biological Breeding, Xinxiang 453003, China
Jiajia Liu
Henan Provincial Key Laboratory of Hybrid Wheat, Henan Institute of Science and Technology, Collaborative Innovation Center of Modern Biological Breeding, Xinxiang 453003, China
Dazhong Zhang
Henan Provincial Key Laboratory of Hybrid Wheat, Henan Institute of Science and Technology, Collaborative Innovation Center of Modern Biological Breeding, Xinxiang 453003, China
Liqiao Jin
Henan Provincial Key Laboratory of Hybrid Wheat, Henan Institute of Science and Technology, Collaborative Innovation Center of Modern Biological Breeding, Xinxiang 453003, China
Nian Yang
Henan Provincial Key Laboratory of Hybrid Wheat, Henan Institute of Science and Technology, Collaborative Innovation Center of Modern Biological Breeding, Xinxiang 453003, China
Bipo Bai
Henan Provincial Key Laboratory of Hybrid Wheat, Henan Institute of Science and Technology, Collaborative Innovation Center of Modern Biological Breeding, Xinxiang 453003, China
Zenghao Wang
Henan Provincial Key Laboratory of Hybrid Wheat, Henan Institute of Science and Technology, Collaborative Innovation Center of Modern Biological Breeding, Xinxiang 453003, China
Suwei Feng
Henan Provincial Key Laboratory of Hybrid Wheat, Henan Institute of Science and Technology, Collaborative Innovation Center of Modern Biological Breeding, Xinxiang 453003, China
Zhengang Ru
Henan Provincial Key Laboratory of Hybrid Wheat, Henan Institute of Science and Technology, Collaborative Innovation Center of Modern Biological Breeding, Xinxiang 453003, China
Tiezhu Hu
Henan Provincial Key Laboratory of Hybrid Wheat, Henan Institute of Science and Technology, Collaborative Innovation Center of Modern Biological Breeding, Xinxiang 453003, China
Nitrogen fertilizer input is the main determinant of wheat yield, and heavy nitrogen fertilizer application causes serious environmental pollution. It is important to understand the genetic response mechanism of wheat to nitrogen and select wheat germplasm with high nitrogen efficiency. In this study, 204 wheat species were used to conduct genome-wide association analysis. Nine phenotypic characteristics were obtained at the seedling stage in hydroponic cultures under low-, normal, and high-nitrogen conditions. A total of 765 significant loci were detected, including 438, 261, and 408 single nucleotide polymorphisms (SNPs) associated with high-, normal, and low-nitrogen conditions, respectively. Among these, 14 SNPs were identified under three conditions, for example, AX-10887638 and AX-94875830, which control shoot length and root–shoot ratio on chromosomes 6A and 6D, respectively. Additionally, 39 SNPs were pleiotropic for multiple traits. Further functional analysis of the genes near the 39 SNPs shows that some candidate genes play key roles in encoding proteins/enzymes, such as transporters, hydrolases, peroxidases, glycosyltransferases, oxidoreductases, acyltransferases, disease-resistant proteins, ubiquitin ligases, and sucrose synthetases. Our results can potentially be used to develop low-nitrogen-tolerant species using marker-assisted selection and provide a theoretical basis for breeding efficient nitrogen-using wheat species.
