ENDOTHELIAL NITRIC OXIDE SYNTHASE GENE G894T POLYMORPHISM IN PATIENTS WITH CORONARY HEART DISEASE AND DIABETES MELLITUS TYPE 2

Abstract

The aim of the study is to estimate the frequency of occurrence of genotypes and alleles of the G894T polymorphic variant of the endothelial nitric oxide synthase (eNOS) gene in patients with chronic coronary heart disease (CHD) and in healthy individuals residing in Grodno region. Material and methods. A total of 135 patients were examined. Among them there were 65 patients with CHD and diabetes mellitus (DМ) type 2 and 70 patients with CHD without DM type 2. The control group included 30 otherwise healthy individuals. Identification of the eNOS gene G894T polymorphism was performed using polymerase chain reaction. Results. Distribution of frequencies of genotypes and alleles of the G894T polymorphic variant of the eNOS gene was revealed in the examined patients as well as in the control group by the results of molecular genetic testing. When determining the frequency of the G894T polymorphic variant of the eNOS gene in the total sample of patients it was established that there were 81 (49.1%) carries of the eNOS 894GG genotype, 63 (38.2%) carries of the heterozygous 894GT genotype, and only 21 (12.7%) patients with the 894TT genotype. Frequency of the 894G allele was 68.2%, while frequency of the 894T allele was 31.8 %. Conclusion. Statistically significant prevalence of the homozygous 894TT genotype of the G894T polymorphic variant of the eNOS gene was established in the 1st group of patients (р=0.01) as well as in the 2nd group (р=0.03) in comparison with the control group. The presence of the 894ТT genotype of the eNOS gene G894T polymorphism increases the risk of CHD by 1.5 times (95% CI 1.29 – 1.74).

Keywords

• genotype
• coronary heart disease
• eNOS gene G894T polymorphism
• diabetes mellitus type 2
• endothelial synthase