Correlative 3D microscopy of single cells using super-resolution and scanning ion-conductance microscopy

Vytautas Navikas; Samuel M. Leitao; Kristin S. Grussmayer; Adrien Descloux; Barney Drake; Klaus Yserentant; Philipp Werther; Dirk-Peter Herten; Richard Wombacher; Aleksandra Radenovic; Georg E. Fantner

doi:10.1038/s41467-021-24901-3

Nature Communications (Jul 2021)

Correlative 3D microscopy of single cells using super-resolution and scanning ion-conductance microscopy

Vytautas Navikas,
Samuel M. Leitao,
Kristin S. Grussmayer,
Adrien Descloux,
Barney Drake,
Klaus Yserentant,
Philipp Werther,
Dirk-Peter Herten,
Richard Wombacher,
Aleksandra Radenovic,
Georg E. Fantner

Affiliations

Vytautas Navikas: Laboratory of Nanoscale Biology, Institute of Bioengineering, School of Engineering, Swiss Federal InstSIitute of Technology Lausanne (EPFL)
Samuel M. Leitao: Laboratory for Bio- and Nano-Instrumentation, Institute of Bioengineering, School of Engineering, Swiss Federal Institute of Technology Lausanne (EPFL)
Kristin S. Grussmayer: Laboratory of Nanoscale Biology, Institute of Bioengineering, School of Engineering, Swiss Federal InstSIitute of Technology Lausanne (EPFL)
Adrien Descloux: Laboratory of Nanoscale Biology, Institute of Bioengineering, School of Engineering, Swiss Federal InstSIitute of Technology Lausanne (EPFL)
Barney Drake: Laboratory for Bio- and Nano-Instrumentation, Institute of Bioengineering, School of Engineering, Swiss Federal Institute of Technology Lausanne (EPFL)
Klaus Yserentant: College of Medical and Dental Sciences, Medical School & School of Chemistry, University of Birmingham
Philipp Werther: Institute of Pharmacy and Molecular Biotechnology, Heidelberg University
Dirk-Peter Herten: College of Medical and Dental Sciences, Medical School & School of Chemistry, University of Birmingham
Richard Wombacher: Institute of Pharmacy and Molecular Biotechnology, Heidelberg University
Aleksandra Radenovic: Laboratory of Nanoscale Biology, Institute of Bioengineering, School of Engineering, Swiss Federal InstSIitute of Technology Lausanne (EPFL)
Georg E. Fantner: Laboratory for Bio- and Nano-Instrumentation, Institute of Bioengineering, School of Engineering, Swiss Federal Institute of Technology Lausanne (EPFL)

DOI: https://doi.org/10.1038/s41467-021-24901-3
Journal volume & issue: Vol. 12, no. 1
pp. 1 – 9

Abstract

Read online

Methods for imaging the 3D cell surface often require physical interaction. Here the authors report the combination of scanning ion conductance microscopy (SICM) and live-cell super-resolution optical fluctuation imaging (SOFI) for the non-invasive topographical imaging of soft biological samples.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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