Frontiers in Materials (Apr 2018)

Hybrid Nanomaterials of Magnetic Iron Nanoparticles and Graphene Oxide as Matrices for the Immobilization of β-Glucosidase: Synthesis, Characterization, and Biocatalytic Properties

  • Georgios Orfanakis,
  • Michaela Patila,
  • Alexandra V. Catzikonstantinou,
  • Kyriaki-Marina Lyra,
  • Antonios Kouloumpis,
  • Konstantinos Spyrou,
  • Petros Katapodis,
  • Alkiviadis Paipetis,
  • Petra Rudolf,
  • Dimitrios Gournis,
  • Haralambos Stamatis

DOI
https://doi.org/10.3389/fmats.2018.00025
Journal volume & issue
Vol. 5

Abstract

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Hybrid nanostructures of magnetic iron nanoparticles and graphene oxide were synthesized and used as nanosupports for the covalent immobilization of β-glucosidase. This study revealed that the immobilization efficiency depends on the structure and the surface chemistry of nanostructures employed. The hybrid nanostructure-based biocatalysts formed exhibited a two to four-fold higher thermostability as compared to the free enzyme, as well as an enhanced performance at higher temperatures (up to 70°C) and in a wider pH range. Moreover, these biocatalysts retained a significant part of their bioactivity (up to 40%) after 12 repeated reaction cycles.

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