Data on the kinetics of in vitro assembled chromatin

Data in Brief. 2016;8:353-359

 

Journal Homepage

Journal Title: Data in Brief

ISSN: 2352-3409 (Online)

Publisher: Elsevier

LCC Subject Category: Medicine: Medicine (General): Computer applications to medicine. Medical informatics | Science: Science (General)

Country of publisher: United States

Language of fulltext: English

Full-text formats available: PDF, HTML

 

AUTHORS

Moritz Carl Völker-Albert (Correspondence to: BioMedical Center, Ludwig-Maximilians-University of Munich, Großhadernerstr. 9, 82152 Planegg-Martinsried, Germany. Tel.: +49 89 2180 75448.; BioMedical Center and Center for Integrated Protein Sciences Munich, Ludwig-Maximilians-University of Munich, Großhaderner Straße 9, 82152 Planegg-Martinsried, Germany)
Miriam Caroline Pusch (BioMedical Center and Center for Integrated Protein Sciences Munich, Ludwig-Maximilians-University of Munich, Großhaderner Straße 9, 82152 Planegg-Martinsried, Germany)
Andreas Schmidt (BioMedical Center and Center for Integrated Protein Sciences Munich, Ludwig-Maximilians-University of Munich, Großhaderner Straße 9, 82152 Planegg-Martinsried, Germany)
Axel Imhof (BioMedical Center and Center for Integrated Protein Sciences Munich, Ludwig-Maximilians-University of Munich, Großhaderner Straße 9, 82152 Planegg-Martinsried, Germany)

EDITORIAL INFORMATION

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Time From Submission to Publication: 4 weeks

 

Abstract | Full Text

Here, we use LC–MS/MS and SWATH-MS to describe the kinetics of in vitro assembled chromatin supported by an embryo extract prepared from preblastoderm Drosophila melanogaster embryos (DREX). This system allows easy manipulation of distinct aspects of chromatin assembly such as post-translational histone modifications, the levels of histone chaperones and the concentration of distinct DNA binding factors. In total, 480 proteins have been quantified as chromatin enriched factors and their binding kinetics have been monitored in the time course of 15 min, 1 h and 4 h of chromatin assembly.The data accompanying the manuscript on this approach, Völker-Albert et al., 2016 “A quantitative proteomic analysis of in vitro assembled chromatin” [1], has been deposited to the ProteomeXchange Consortium (http://www.proteomexchange.org) via the PRIDE partner repository with the dataset identifier submission number PRIDE: PXD002537 and PRIDE: PXD003445. Keywords: chromatin assembly, quantitative proteomics, in-vitro, Drosophila melanogaster