Evaluation of the synergistic effects of synthesized silver nanoparticles conjugated tetracycline

Abstract

The antibiotic impacts of classical drugs against bacteria could be enhanced via the conjugation of silver nanoparticles. After the successful coating of silver nanoparticles (AgNPs) with Tetracycline, the characterization of the conjugate properties was achieved through measurements with scanning electron microscopy (SEM) coupled to an energy-dispersive X-ray analyzer (EDX). The SEM analysis shows the appearance of silver nanoparticles with an average particle size of 22.82 nm and a cubic shape. Meanwhile, the EDX spectrum of silver NPs exhibits peaks corresponding to elemental silver. The anti-bacterial activity of pure Tetracycline and tetracycline-AgNP conjugates was examined against ten isolates of Pseudomonas aeruginosa. The tetracycline/AgNPs nanoparticles also strongly inhibited the growth of P. aeruginosa, as shown in experiments that involved the determination of the lowest inhibitory and lethal concentrations. It is noted that all of the isolates recorded the minimum inhibitory concentration at 60%, while the minimum inhibitory concentration for two isolates (1 and 10) was 100%. Genetically, higher frequencies of total chromosomal aberrations (TCAs) in blood cells were correlated with higher NP concentrations. The tetracycline-AgNPs effectively scavenged the 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radicals. Toxicity tests against MCF-7 cancer cells revealed an antitumor effect of NPs against cancer cells, showing the capability of inhibiting the proliferation of cells and demonstrating highly significant effects. 

Keywords

• antibiotics nanoparticles anti
• bacterial activity mic pathogenic bacteria