Bio-Protocol
(Aug 2013)
Assay of Blood Brain Barrier and Placental Barrier Permeability
Saurabh Agnihotri,
Poonam Singh,
Balawant Kumar,
Pankaj Singh,
Swatantra Jain,
Mahesh ChandraTewari,
Sadan Kumar,
Monika Sachdev,
Raj Tripathi
Affiliations
Saurabh Agnihotri
Endocrinology Division, Central Drug Research Institute (Council of Scientific and Industrial Research), Lucknow, India
Poonam Singh
Toxicology Division, Central Drug Research Institute (Council of Scientific and Industrial Research), Lucknow, India
Balawant Kumar
Toxicology Division, Central Drug Research Institute (Council of Scientific and Industrial Research), Lucknow, India
Pankaj Singh
Endocrinology Division, Central Drug Research Institute (Council of Scientific and Industrial Research), Lucknow, India
Swatantra Jain
Endocrinology Division, Central Drug Research Institute (Council of Scientific and Industrial Research), Lucknow, India
Mahesh ChandraTewari
Endocrinology Division, Central Drug Research Institute (Council of Scientific and Industrial Research), Lucknow, India
Sadan Kumar
Toxicology Division, Central Drug Research Institute (Council of Scientific and Industrial Research), Lucknow, India
Monika Sachdev
Endocrinology Division, Central Drug Research Institute (Council of Scientific and Industrial Research), Lucknow, India
Raj Tripathi
Toxicology Division, Central Drug Research Institute (Council of Scientific and Industrial Research), Lucknow, India
DOI
https://doi.org/10.21769/BioProtoc.845
Journal volume & issue
Vol. 3,
no. 15
Abstract
Read online
Evans blue dye solution was used to observe the effect of a viral protein on two pre-defined barriers of the body i.e. the blood brain barrier (BBB) and the placental barrier (PB). This dye has strong affinity for serum albumin and does not cross these barriers under natural conditions. As all the dye gets bound to albumin, all the neural tissues and embryonic tissues remain unstained. When the BBB and PB are compromised due to the breach of these barriers, albumin-bound Evans blue enters the CNS and the placenta.
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