Study of the Effect of the Pharmaceutical Substance Branched Oligohexamethylene Guanidine Hydrosuccinate in Relation to Microorganisms

Abstract

The acquisition of resistance to the antibiotics and antibacterial agents used by microorganisms sets the task of finding new active disinfectants. It is known that compounds of the oligoguanidine class possess pronounced antibacterial properties, low toxicity, and can exhibit a prolonged biocidal effect, causing destruction of biofilms formed by pathogenic microflora. One of these compounds is branched oligohexamethylene guanidine hydrosuccinate (OHMG succ). The specified compound can be used as an active component in the development of a drug that acts on microbial dehydrogenases for the prevention and treatment of conjunctivitis of an infectious nature. In this regard, the purpose of this work is to study and visualize the mechanism of action of OHMG succ in relation to microbial dehydrogenases of Gram-negative bacteria Escherichia coli and Pseudomonas aeruginosa by colorimetric methods, to study its effect on the formation of biofilms and mature biofilms of E.coli, on its cell wall by scanning electron microscopy, as well as experimental evidence of the influence of OHMG succ on the morphology of several types of bacteria, yeasts, and molds that cause conjunctivitis, by atomic force microscopy. In addition, it was necessary to identify the dependence of cell segregation disturbance on the increasing concentration of OHMG succ. Thus, it was proved that OHMG succ affects not only the microbial dehydrogenases of certain Gram-negative bacteria, the mature biofilm of E.coli, but also its cell wall. The effect of OHMG succ on the morphology of several types of bacteria, yeast and mold fungi was also revealed. The indicated information shows that the creation of an antimicrobial drug based on OHMG succ is of interest, and this work may serve as the basis for the development of an innovative pharmacological drug for the treatment of conjunctivitis of an infectious nature.

Keywords

• antibacterial agent
• biofilm
• conjunctivitis
• mechanism of action
• microbial dehydrogenases
• ophthalmology
• pathogens