Association of Alpha Tocopherol and Ag Sulfadiazine Chitosan Oleate Nanocarriers in Bioactive Dressings Supporting Platelet Lysate Application to Skin Wounds
Maria Cristina Bonferoni,
Giuseppina Sandri,
Silvia Rossi,
Eleonora Dellera,
Alessandro Invernizzi,
Cinzia Boselli,
Antonia Icaro Cornaglia,
Claudia Del Fante,
Cesare Perotti,
Barbara Vigani,
Federica Riva,
Carla Caramella,
Franca Ferrari
Affiliations
Maria Cristina Bonferoni
Department of Drug Sciences, University of Pavia, 27100 Pavia, Italy
Giuseppina Sandri
Department of Drug Sciences, University of Pavia, 27100 Pavia, Italy
Silvia Rossi
Department of Drug Sciences, University of Pavia, 27100 Pavia, Italy
Eleonora Dellera
Department of Drug Sciences, University of Pavia, 27100 Pavia, Italy
Alessandro Invernizzi
Department of Drug Sciences, University of Pavia, 27100 Pavia, Italy
Cinzia Boselli
Department of Drug Sciences, University of Pavia, 27100 Pavia, Italy
Antonia Icaro Cornaglia
Department of Public Health Experimental and Forensic Medicine, University of Pavia, 27100 Pavia, Italy
Claudia Del Fante
Immunohaematology and Transfusion Service and Cell Therapy Unit of Fondazione IRCCS, S. Matteo, 27100 Pavia, Italy
Cesare Perotti
Immunohaematology and Transfusion Service and Cell Therapy Unit of Fondazione IRCCS, S. Matteo, 27100 Pavia, Italy
Barbara Vigani
Department of Drug Sciences, University of Pavia, 27100 Pavia, Italy
Federica Riva
Department of Public Health Experimental and Forensic Medicine, University of Pavia, 27100 Pavia, Italy
Carla Caramella
Department of Drug Sciences, University of Pavia, 27100 Pavia, Italy
Franca Ferrari
Department of Drug Sciences, University of Pavia, 27100 Pavia, Italy
Chitosan oleate was previously proposed to encapsulate in nanocarriers some poorly soluble molecules aimed to wound therapy, such as the anti-infective silver sulfadiazine, and the antioxidant α tocopherol. Because nanocarriers need a suitable formulation to be administered to wounds, in the present paper, these previously developed nanocarriers were loaded into freeze dried dressings based on chitosan glutamate. These were proposed as bioactive dressings aimed to support the application to wounds of platelet lysate, a hemoderivative rich in growth factors. The dressings were characterized for hydration capacity, morphological aspect, and rheological and mechanical behavior. Although chitosan oleate nanocarriers clearly decreased the mechanical properties of dressings, these remained compatible with handling and application to wounds. Preliminary studies in vitro on fibroblast cell cultures demonstrated good compatibility of platelet lysate with nanocarriers and bioactive dressings. An in vivo study on a murine wound model showed an accelerating wound healing effect for the bioactive dressing and its suitability as support of the platelet lysate application to wounds.
