Diversity of Catechin Content in the Leaves of Persimmon Germplasms

Yiru Wang; Yujing Suo; Huawei Li; Weijuan Han; Peng Sun; Fangdong Li; Jianmin Fu

doi:10.3390/horticulturae9040464

Horticulturae (Apr 2023)

Diversity of Catechin Content in the Leaves of Persimmon Germplasms

Yiru Wang,
Yujing Suo,
Huawei Li,
Weijuan Han,
Peng Sun,
Fangdong Li,
Jianmin Fu

Affiliations

Yiru Wang: Research Institute of Non-Timber Forestry, Chinese Academy of Forestry, Zhengzhou 450003, China
Yujing Suo: Research Institute of Non-Timber Forestry, Chinese Academy of Forestry, Zhengzhou 450003, China
Huawei Li: Research Institute of Non-Timber Forestry, Chinese Academy of Forestry, Zhengzhou 450003, China
Weijuan Han: Research Institute of Non-Timber Forestry, Chinese Academy of Forestry, Zhengzhou 450003, China
Peng Sun: Research Institute of Non-Timber Forestry, Chinese Academy of Forestry, Zhengzhou 450003, China
Fangdong Li: Research Institute of Non-Timber Forestry, Chinese Academy of Forestry, Zhengzhou 450003, China
Jianmin Fu: Research Institute of Non-Timber Forestry, Chinese Academy of Forestry, Zhengzhou 450003, China

DOI: https://doi.org/10.3390/horticulturae9040464
Journal volume & issue: Vol. 9, no. 4
p. 464

Abstract

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Catechins are essential phenolic compounds in persimmon. However, the catechin content in the leaves of persimmon germplasms has not been systematically and comprehensively evaluated. To systematically assess catechin variation in different growth stages and astringency types of persimmon leaves, the young and adult leaves catechin content of 249 persimmon germplasms from Korea, Japan, and 16 provinces in China was quantified using an HPLC method. The results showed that the content of (−)-epicatechin (EC) and (−)-gallocatechin gallate (GCG) had higher coefficient of variation (CV) values in persimmon young leaves (106.74%) and adult leaves (91.49%), respectively. The genetic diversity H’ of (+)-catechin (CA) and (+)-gallocatechin (GC) content was higher in young leaves (2.00 and 2.03), and the H’ value of (−)-epigallocatechin (EGC) and CA content was higher in adult leaves (1.98 and 1.92). The non-galloylated catechins, EGC, EC, and CA, were significantly positively correlated with each other in both young and adult leaves. Catechins of persimmon leaves showed different accumulation patterns in different growth stages and astringency types; (−)-epigallocatechin gallate (EGCG) and CA had the highest average content in the persimmon germplasms of Guangxi and Shanxi provinces of China, respectively. The content of (+)-catechin gallate (CG) was found to be significantly higher in Japanese pollination-constant non-astringent (J-PCNA) persimmon leaves, and EC, GCG had the highest levels in Chinese PCNA (C-PCNA) type. The 249 germplasms were classified into five clusters (Cluster I-V) by SOM clustering based on the content of nine catechins, with EC, CA, and GC having the highest content in Cluster V of the adult leaves. In addition, four excellent germplasms of Cluster III had the highest EGC and GC content in young leaves. Thus, the critical period for catechin utilization of persimmon leaves can be further determined, and provide theoretical references for excellent germplasm screening.

Published in Horticulturae

ISSN: 2311-7524 (Online)
Publisher: MDPI AG
Country of publisher: Switzerland
LCC subjects: Agriculture: Plant culture
Website: http://www.mdpi.com/journal/horticulturae

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