International Journal of Nanomedicine (Jun 2020)
Nanoparticles of Antroquinonol-Rich Extract from Solid-State-Cultured Antrodia cinnamomea Improve Reproductive Function in Diabetic Male Rats
Abstract
Zwe-Ling Kong,1 Jia-Ling He,1 Sabri Sudirman,2 Mao-Tien Kuo,3 Song Miao,4 Ke-Liang B Chang,1 David Tsou1 1Department of Food Science, National Taiwan Ocean University, Keelung City, Taiwan; 2Fisheries Product Technology, Faculty of Agriculture, Universitas Sriwijaya, Palembang, Ogan Ilir Regency, Indonesia; 3Lantyng Biotech, Co. Ltd., Taipei City, Taiwan; 4Teagasc Food Research Center, Moorepark, Co. Cork, IrelandCorrespondence: Zwe-Ling Kong Tel +886-24622192 #5130 Email [email protected]: To characterize the nanoparticle of antroquinonol from A. cinnamomea and its ameliorative effects on the reproductive dysfunction in the diabetic male rat.Material and Methods: The chitosan-silicate nanoparticle was used as the carrier for the delivery of antroquinonol from solid-state-cultured A. cinnamomea extract (AC). The rats were fed with a high-fat diet and intraperitoneally injected with streptozotocin to induce diabetes. The rats were daily oral gavage by water [Diabetes (DM) and Control groups], three different doses of chitosan-silicate nanoparticle of antroquinonol from solid-state-cultured A. cinnamomea (nano-SAC, NAC): (DM+NAC1x, 4 mg/kg of body weight; DM+NAC2x, 8 mg/kg; and DM+NAC5x, 20 mg/kg), solid-state-cultured AC (DM+AC5x, 20 mg/kg), or metformin (DM+Met, 200 mg/kg) for 7 weeks.Results: The nano-SAC size was 37.68± 5.91 nm, the zeta potential was 4.13± 0.49 mV, encapsulation efficiency was 79.29± 0.77%, and loading capacity was 32.45± 0.02%. The nano-SAC can improve diabetes-induced reproductive dysfunction by regulating glucose, insulin, and oxidative enzyme and by increasing the level of testosterone, follicle-stimulating hormone, luteinizing hormone, and sperm count as well as sperm mobility. In testicular histopathology, the seminiferous tubules of A. cinnamomea-supplemented diabetic rats showed similar morphology with the control group.Conclusion: The nanoparticle of antroquinonol from Antrodia cinnamomea can be used as an effective strategy to improve diabetes-induced testicular dysfunction.Keywords: Antrodia cinnamomea, antroquinonol, diabetes, nanoparticles, male reproduction
