Methylation of recombinant mononucleosomes by DNMT3A demonstrates efficient linker DNA methylation and a role of H3K36me3

Alexander Bröhm; Tabea Schoch; Michael Dukatz; Nora Graf; Franziska Dorscht; Evelin Mantai; Sabrina Adam; Pavel Bashtrykov; Albert Jeltsch

doi:10.1038/s42003-022-03119-z

Communications Biology (Mar 2022)

Methylation of recombinant mononucleosomes by DNMT3A demonstrates efficient linker DNA methylation and a role of H3K36me3

Alexander Bröhm,
Tabea Schoch,
Michael Dukatz,
Nora Graf,
Franziska Dorscht,
Evelin Mantai,
Sabrina Adam,
Pavel Bashtrykov,
Albert Jeltsch

Affiliations

Alexander Bröhm: Department of Biochemistry, Institute of Biochemistry and Technical Biochemistry, University of Stuttgart
Tabea Schoch: Department of Biochemistry, Institute of Biochemistry and Technical Biochemistry, University of Stuttgart
Michael Dukatz: Department of Biochemistry, Institute of Biochemistry and Technical Biochemistry, University of Stuttgart
Nora Graf: Department of Biochemistry, Institute of Biochemistry and Technical Biochemistry, University of Stuttgart
Franziska Dorscht: Department of Biochemistry, Institute of Biochemistry and Technical Biochemistry, University of Stuttgart
Evelin Mantai: Department of Biochemistry, Institute of Biochemistry and Technical Biochemistry, University of Stuttgart
Sabrina Adam: Department of Biochemistry, Institute of Biochemistry and Technical Biochemistry, University of Stuttgart
Pavel Bashtrykov: Department of Biochemistry, Institute of Biochemistry and Technical Biochemistry, University of Stuttgart
Albert Jeltsch: Department of Biochemistry, Institute of Biochemistry and Technical Biochemistry, University of Stuttgart

DOI: https://doi.org/10.1038/s42003-022-03119-z
Journal volume & issue: Vol. 5, no. 1
pp. 1 – 12

Abstract

Read online

DNA methyltransferase 3A (DNMT3A) is shown to efficiently methylate recombinant mononucleosome linker DNA and a role for H3K36me3 in the stimulation of DNA methylation is revealed that is independent of its interaction with the PWWP domain.

Published in Communications Biology

ISSN: 2399-3642 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science: Biology (General)
Website: https://www.nature.com/commsbio/

About the journal