Effect of food plants on Spodoptera litura (Lepidoptera: Noctuidae) larvae immune and antioxidant properties in response to Bacillus thuringiensis infection

Abstract

The larvae of Spodoptera litura (Fabricius) were reared on five host plants, Brassica oleracea, Nicotiana tabacum, Ricinus communis, Gossypium hirsutum, and Arachis hypogaea. The larvae were immunized with Bacillus thuringiensis to observe the immune response. The results of total and differential hemocyte count were increased in B. oleracea, N. tabacum, and R. communis fed S. litura larval hemolymph. Similar results were observed in the parameter of nodulation, melanization, and phenoloxidase. Total protein was higher in R. communis fed larvae. Antioxidant levels like Catalase (CAT), Superoxide dismutase (SOD), Glutathione S- transferase (GST), Peroxidase (POX), Lipid peroxidase (LPO), and Esterase (EST) was found in moreover all plant-feeding insect. High CAT activity was observed 2–6 h in R. communis, G. hirsutum, and A. hypogaea fed S. litura larval midgut and fatbody samples. Increased SOD activity in both midgut and fatbody at 2–12 h of B. oleracea, G. hirsutum, and A. hypogaea fed. GST activity was increased initially 2−6 h in G. hirsutum and A. hypogaea. Increased POX activity was observed initially in all treated groups. Highest LPO observed at 6 h in N. tabacum in both midgut and fatbody. Whereas increased EST activity was observed in N. tabacum and B. oleracea. The results of the present study shows that nature of food influence the immunity against Bt infection. This information can be very useful for incorporating biological control program for insect pest.

Keywords

• Host plant
• Spodoptera litura
• Bacillus thuringiensis
• Immunity
• Antioxidant
• Detoxification