Cultivation and application of nicotine-degrading bacteria and environmental functioning in tobacco planting soil

Abstract

Highlights Chitinophaga spp. and Flavobacterium spp. were first confirmed as NDB. 52 NDB strains from seven genera were isolated from tobacco rhizosphere soil. Strains ND6 and ND16 efficiently degraded nicotine in medium and tobacco waste compost. ND16 may have a new nicotine-degrading pathway by phenotype and genome analysis. Abundance of five nicotine-degrading genes in soil correlated well with nicotine concentrations.

Keywords

• Nicotine-degrading bacteria (NDB)
• Isolation
• Metabolic pathway
• Metagenomics
• Compost of tobacco waste