Expression Patterns, Molecular Characterization, and Response to Host Stress of CYP Genes from <i>Phenacoccus solenopsis</i> (Hemiptera: Pseudococcidae)
Lingyu Xi,
Dan Liu,
Lei Ma,
Ying Zhang,
Ruirui Sheng,
Shaobing Zhang,
Xiangli Dang,
Guiting Li,
Yong Miao,
Junqi Jiang
Affiliations
Lingyu Xi
Department of Entomology, College of Plant Protection, Anhui Agricultural University, Hefei 230036, China
Dan Liu
Department of Entomology, College of Plant Protection, Anhui Agricultural University, Hefei 230036, China
Lei Ma
Department of Entomology, College of Plant Protection, Anhui Agricultural University, Hefei 230036, China
Ying Zhang
Department of Entomology, College of Plant Protection, Anhui Agricultural University, Hefei 230036, China
Ruirui Sheng
Department of Entomology, College of Plant Protection, Anhui Agricultural University, Hefei 230036, China
Shaobing Zhang
Department of Entomology, College of Plant Protection, Anhui Agricultural University, Hefei 230036, China
Xiangli Dang
Department of Entomology, College of Plant Protection, Anhui Agricultural University, Hefei 230036, China
Guiting Li
Department of Entomology, College of Plant Protection, Anhui Agricultural University, Hefei 230036, China
Yong Miao
Department of Entomology, College of Plant Protection, Anhui Agricultural University, Hefei 230036, China
Junqi Jiang
Department of Entomology, College of Plant Protection, Anhui Agricultural University, Hefei 230036, China
The quarantine insect pest Phenacoccus solenopsis (Hemiptera: Pseudococcidae) has a broad host range and is distributed worldwide. Each year, P. solenopsis causes significant crop losses. The detoxification of various xenobiotic compounds involves the cytochrome P450 monooxygenase (CYP) superfamily of enzymes. However, the functions of CYPs in P. solenopsis are poorly understood. In the present study, P. solenopsis was reared from the egg to the adult stage on three host plants: Tomato, cotton, and hibiscus. Thirty-seven P. solenopsis CYP genes were identified and their phylogenetic relationships were analyzed. Eleven CYP genes (PsCYP4NT1, PsCYP4G219, PsCYP6PZ1, PsCYP6PZ5, PsCYP301B1, PsCYP302A1, PsCYP305A22, PsCYP315A1, PsCYP353F1, PsCYP3634A1, and PsCYP3635A2) were selected for quantitative real-time PCR analysis. The results demonstrated marked differences in CYP expression levels in P. solenopsis grown on different host plants. The results will aid the molecular characterization of CYPs and will increase our understanding of CYP expression patterns in P. solenopsis during development and growth on different hosts.
