Nature Communications (Oct 2018)

Probing aggrephagy using chemically-induced protein aggregates

  • Anne F. J. Janssen,
  • Eugene A. Katrukha,
  • Wendy van Straaten,
  • Pauline Verlhac,
  • Fulvio Reggiori,
  • Lukas C. Kapitein

DOI
https://doi.org/10.1038/s41467-018-06674-4
Journal volume & issue
Vol. 9, no. 1
pp. 1 – 10

Abstract

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Autophagic clearance of aggregates, aggrephagy, is essential for cellular homeostasis but tools to induce and monitor it are limited. Here the authors present a fluorescence-based aggrephagy induction assay to study spatiotemporal dynamics and control mechanisms driving protein aggregate clearance.