Genotoxic and Cytotoxic Properties of Zinc Oxide Nanoparticles Phyto-Fabricated from the Obscure Morning Glory Plant <i>Ipomoea obscura</i> (L.) Ker Gawl
Mahadevamurthy Murali,
Satish Anandan,
Mohammad Azam Ansari,
Mohammad A. Alzohairy,
Mohammad N. Alomary,
Sarah Mousa Maadi Asiri,
Ahmad Almatroudi,
M. C. Thriveni,
Sudarshana Brijesh Singh,
Hittanahallikoppal Gajendramurthy Gowtham,
Mohammed Aiyaz,
Chandrashekar Srinivasa,
Asna Urooj,
Kestur Nagaraj Amruthesh
Affiliations
Mahadevamurthy Murali
Applied Plant Pathology Laboratory, Department of Studies in Botany, University of Mysore, Manasagangotri, Mysore 570006, Karnataka, India
Satish Anandan
Department of Studies in Food Science and Nutrition, University of Mysore, Manasagangotri, Mysore 570006, Karnataka, India
Mohammad Azam Ansari
Department of Epidemic Disease Research, Institutes for Research and Medical Consultations (IRMC), Imam Abdulrahman Bin Faisal University, Dammam 31441, Saudi Arabia
Mohammad A. Alzohairy
Department of Medical Laboratories, College of Applied Medical Sciences, Qassim University, Qassim 51431, Saudi Arabia
Mohammad N. Alomary
National Center for Biotechnology, Life Science and Environmental Research Institute, King Abdulaziz City for Science and Technology, Riyadh P.O. Box 6086, Riyadh 11442, Saudi Arabia
Sarah Mousa Maadi Asiri
Department of Biophysics, Institute for Research and Medical Consultations (IRMC), Imam Abdulrahman Bin Faisal University, Dammam 31441, Saudi Arabia
Ahmad Almatroudi
Department of Medical Laboratories, College of Applied Medical Sciences, Qassim University, Qassim 51431, Saudi Arabia
M. C. Thriveni
Central Sericultural Germplasm Resources Centre, Central Silk Board, Ministry of Textiles, Thally Road, TVS Nagar, Hosur 635109, Tamil Nadu, India
Sudarshana Brijesh Singh
Department of Studies in Biotechnology, University of Mysore, Manasagangotri, Mysore 570006, Karnataka, India
Hittanahallikoppal Gajendramurthy Gowtham
Department of Studies in Biotechnology, University of Mysore, Manasagangotri, Mysore 570006, Karnataka, India
Mohammed Aiyaz
Department of Studies in Biotechnology, University of Mysore, Manasagangotri, Mysore 570006, Karnataka, India
Chandrashekar Srinivasa
Department of Studies in Biotechnology, Davangere University, Davangere 577007, Karnataka, India
Asna Urooj
Department of Studies in Food Science and Nutrition, University of Mysore, Manasagangotri, Mysore 570006, Karnataka, India
Kestur Nagaraj Amruthesh
Applied Plant Pathology Laboratory, Department of Studies in Botany, University of Mysore, Manasagangotri, Mysore 570006, Karnataka, India
The study was undertaken to investigate the antioxidant, genotoxic, and cytotoxic potentialities of phyto-fabricated zinc oxide nanoparticles (ZnO-NPs) from Ipomoea obscura (L.) Ker Gawl. aqueous leaf extract. The UV-visible spectral analysis of the ZnO-NPs showed an absorption peak at 304 nm with a bandgap energy of 3.54 eV, which are characteristics of zinc nanoparticles. Moreover, the particles were of nano-size (~24.26 nm) with 88.11% purity and were agglomerated as observed through Scanning Electron Microscopy (SEM). The phyto-fabricated ZnO-NPs offered radical scavenging activity (RSA) in a dose-dependent manner with an IC50 of 0.45 mg mL−1. In addition, the genotoxicity studies of ZnO-NPs carried out on onion root tips revealed that the particles were able to significantly inhibit the cell division at the mitotic stage with a mitotic index of 39.49%. Further, the cytotoxic studies on HT-29 cells showed that the phyto-fabricated ZnO-NPs could arrest the cell division as early as in the G0/G1 phase (with 92.14%) with 73.14% cells showing early apoptotic symptoms after 24 h of incubation. The results of the study affirm the ability of phyto-fabricated ZnO-NPs from aqueous leaf extract of I. obscura is beneficial in the cytotoxic application.
