International Journal of Nanomedicine (Feb 2020)

Electrospraying Oxygen-Generating Microparticles for Tissue Engineering Applications

  • Morais AIS,
  • Wang X,
  • Vieira EG,
  • Viana BC,
  • Silva-Filho EC,
  • Osajima JA,
  • Afewerki S,
  • Corat MAF,
  • Silva HS,
  • Marciano FR,
  • Ruiz-Esparza GU,
  • Stocco TD,
  • de Paula MMM,
  • Lobo AO

Journal volume & issue
Vol. Volume 15
pp. 1173 – 1186

Abstract

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Alan IS Morais, 1,* Xichi Wang, 2–4,* Ewerton G Vieira, 1 Bartolomeu C Viana, 1, 5 Edson C Silva-Filho, 1 Josy A Osajima, 1 Samson Afewerki, 3, 4 Marcus AF Corat, 6 Heurison S Silva, 5 Fernanda R Marciano, 5 Guillermo U Ruiz-Esparza, 3, 4 Thiago D Stocco, 6, 7 Mirian MM de Paula, 6 Anderson O Lobo 1 1LIMAV-Interdisciplinary Laboratory for Advanced Materials, Materials Science and Engineering Graduate Program, UFPI-Federal University of Piauí, Teresina, PI CEP 64049-550, Brazil; 2Department of Cardiovascular Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, People’s Republic of China; 3Division of Engineering in Medicine, Department of Medicine, Harvard Medical School, Brigham & Women´s Hospital, Cambridge, MA 02139, USA; 4Harvard-MIT Division of Health Science and Technology, Massachusetts Institute of Technology, MIT, Cambridge, MA 02139, USA; 5Department of Physics, UFPI-Federal University of Piauí, Teresina, PI CEP 64049-550, Brazil; 6Multidisciplinary Center for Biological Research, University of Campinas (UNICAMP), Campinas 13083-877, Brazil; 7Faculty of Physiotherapy, Santo Amaro University, São Paulo 04829-300, Brazil*These authors contributed equally to this workCorrespondence: Anderson O Lobo Email [email protected]: The facile preparation of oxygen-generating microparticles (M) consisting of Polycaprolactone (PCL), Pluronic F-127, and calcium peroxide (CPO) (PCL-F-CPO-M) fabricated through an electrospraying process is disclosed. The biological study confirmed the positive impact from the oxygen-generating microparticles on the cell growth with high viability. The presented technology could work as a prominent tool for various tissue engineering and biomedical applications.Methods: The oxygen-generated microparticles fabricated through electrospraying processes were thoroughly characterization through various methods such as X-ray diffraction (XRD), Fourier Transform Infrared Spectroscopy (FTIR) analysis, and scanning electron microscopy (SEM)/SEM-Energy Dispersive Spectroscopy (EDS) analysis.Results: The analyses confirmed the presence of the various components and the porous structure of the microparticles. Spherical shape with spongy characteristic microparticles were obtained with negative charge surface (ζ = – 16.9) and a size of 17.00 ± 0.34 μm. Furthermore, the biological study performed on rat chondrocytes demonstrated good cell viability and the positive impact of increasing the amount of CPO in the PCL-F-CPO-M.Conclusion: This technological platform could work as an important tool for tissue engineering due to the ability of the microparticles to release oxygen in a sustained manner for up to 7 days with high cell viability.Keywords: oxygen-generating-microparticles, electrospraying, tissue engineering, calcium peroxide, cartilage

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