Frontiers in Cell and Developmental Biology (Sep 2021)
Adipose/Connective Tissue From Thyroid-Associated Ophthalmopathy Uncovers Interdependence Between Methylation and Disease Pathogenesis: A Genome-Wide Methylation Analysis
- Yu Liang,
- Yu Liang,
- Yu Liang,
- Yu Liang,
- Sijia Ding,
- Xiying Wang,
- Xiying Wang,
- Xiying Wang,
- Xiying Wang,
- Chunchun Hu,
- Chunchun Hu,
- Chunchun Hu,
- Chunchun Hu,
- Yihan Zhang,
- Yihan Zhang,
- Yihan Zhang,
- Yihan Zhang,
- Yan Hu,
- Yan Hu,
- Yan Hu,
- Yan Hu,
- Yuye Zhang,
- Yuye Zhang,
- Yuye Zhang,
- Yuye Zhang,
- Hongyu Kong,
- Hongyu Kong,
- Hongyu Kong,
- Hongyu Kong,
- Weiyi Xia,
- Weiyi Xia,
- Weiyi Xia,
- Weiyi Xia,
- Qinghe Jing,
- Qinghe Jing,
- Qinghe Jing,
- Qinghe Jing,
- Yuxiang Hu,
- Yuxiang Hu,
- Yuxiang Hu,
- Yuxiang Hu,
- Chen Zhao,
- Chen Zhao,
- Chen Zhao,
- Chen Zhao,
- Lianqun Wu,
- Lianqun Wu,
- Lianqun Wu,
- Lianqun Wu
Affiliations
- Yu Liang
- Eye Institute and Department of Ophthalmology, Eye and ENT Hospital, Fudan University, Shanghai, China
- Yu Liang
- NHC Key Laboratory of Myopia (Fudan University), Shanghai, China
- Yu Liang
- Key Laboratory of Myopia, Chinese Academy of Medical Sciences, Shanghai, China
- Yu Liang
- Shanghai Key Laboratory of Visual Impairment and Restoration, Shanghai, China
- Sijia Ding
- Department of Phase 1 Clinical Trial Unit, The First Affiliated Hospital with Nanjing Medical University, Nanjing, China
- Xiying Wang
- Eye Institute and Department of Ophthalmology, Eye and ENT Hospital, Fudan University, Shanghai, China
- Xiying Wang
- NHC Key Laboratory of Myopia (Fudan University), Shanghai, China
- Xiying Wang
- Key Laboratory of Myopia, Chinese Academy of Medical Sciences, Shanghai, China
- Xiying Wang
- Shanghai Key Laboratory of Visual Impairment and Restoration, Shanghai, China
- Chunchun Hu
- Eye Institute and Department of Ophthalmology, Eye and ENT Hospital, Fudan University, Shanghai, China
- Chunchun Hu
- NHC Key Laboratory of Myopia (Fudan University), Shanghai, China
- Chunchun Hu
- Key Laboratory of Myopia, Chinese Academy of Medical Sciences, Shanghai, China
- Chunchun Hu
- Shanghai Key Laboratory of Visual Impairment and Restoration, Shanghai, China
- Yihan Zhang
- Eye Institute and Department of Ophthalmology, Eye and ENT Hospital, Fudan University, Shanghai, China
- Yihan Zhang
- NHC Key Laboratory of Myopia (Fudan University), Shanghai, China
- Yihan Zhang
- Key Laboratory of Myopia, Chinese Academy of Medical Sciences, Shanghai, China
- Yihan Zhang
- Shanghai Key Laboratory of Visual Impairment and Restoration, Shanghai, China
- Yan Hu
- Eye Institute and Department of Ophthalmology, Eye and ENT Hospital, Fudan University, Shanghai, China
- Yan Hu
- NHC Key Laboratory of Myopia (Fudan University), Shanghai, China
- Yan Hu
- Key Laboratory of Myopia, Chinese Academy of Medical Sciences, Shanghai, China
- Yan Hu
- Shanghai Key Laboratory of Visual Impairment and Restoration, Shanghai, China
- Yuye Zhang
- Eye Institute and Department of Ophthalmology, Eye and ENT Hospital, Fudan University, Shanghai, China
- Yuye Zhang
- NHC Key Laboratory of Myopia (Fudan University), Shanghai, China
- Yuye Zhang
- Key Laboratory of Myopia, Chinese Academy of Medical Sciences, Shanghai, China
- Yuye Zhang
- Shanghai Key Laboratory of Visual Impairment and Restoration, Shanghai, China
- Hongyu Kong
- Eye Institute and Department of Ophthalmology, Eye and ENT Hospital, Fudan University, Shanghai, China
- Hongyu Kong
- NHC Key Laboratory of Myopia (Fudan University), Shanghai, China
- Hongyu Kong
- Key Laboratory of Myopia, Chinese Academy of Medical Sciences, Shanghai, China
- Hongyu Kong
- Shanghai Key Laboratory of Visual Impairment and Restoration, Shanghai, China
- Weiyi Xia
- Eye Institute and Department of Ophthalmology, Eye and ENT Hospital, Fudan University, Shanghai, China
- Weiyi Xia
- NHC Key Laboratory of Myopia (Fudan University), Shanghai, China
- Weiyi Xia
- Key Laboratory of Myopia, Chinese Academy of Medical Sciences, Shanghai, China
- Weiyi Xia
- Shanghai Key Laboratory of Visual Impairment and Restoration, Shanghai, China
- Qinghe Jing
- Eye Institute and Department of Ophthalmology, Eye and ENT Hospital, Fudan University, Shanghai, China
- Qinghe Jing
- NHC Key Laboratory of Myopia (Fudan University), Shanghai, China
- Qinghe Jing
- Key Laboratory of Myopia, Chinese Academy of Medical Sciences, Shanghai, China
- Qinghe Jing
- Shanghai Key Laboratory of Visual Impairment and Restoration, Shanghai, China
- Yuxiang Hu
- Eye Institute and Department of Ophthalmology, Eye and ENT Hospital, Fudan University, Shanghai, China
- Yuxiang Hu
- NHC Key Laboratory of Myopia (Fudan University), Shanghai, China
- Yuxiang Hu
- Key Laboratory of Myopia, Chinese Academy of Medical Sciences, Shanghai, China
- Yuxiang Hu
- Shanghai Key Laboratory of Visual Impairment and Restoration, Shanghai, China
- Chen Zhao
- Eye Institute and Department of Ophthalmology, Eye and ENT Hospital, Fudan University, Shanghai, China
- Chen Zhao
- NHC Key Laboratory of Myopia (Fudan University), Shanghai, China
- Chen Zhao
- Key Laboratory of Myopia, Chinese Academy of Medical Sciences, Shanghai, China
- Chen Zhao
- Shanghai Key Laboratory of Visual Impairment and Restoration, Shanghai, China
- Lianqun Wu
- Eye Institute and Department of Ophthalmology, Eye and ENT Hospital, Fudan University, Shanghai, China
- Lianqun Wu
- NHC Key Laboratory of Myopia (Fudan University), Shanghai, China
- Lianqun Wu
- Key Laboratory of Myopia, Chinese Academy of Medical Sciences, Shanghai, China
- Lianqun Wu
- Shanghai Key Laboratory of Visual Impairment and Restoration, Shanghai, China
- DOI
- https://doi.org/10.3389/fcell.2021.716871
- Journal volume & issue
-
Vol. 9
Abstract
In response to pathological stimulation, methylation status conversion of the genome drives changes of cell feature and is able to promote disease development. Yet the role of methylation in the development of thyroid-associated ophthalmopathy (TAO) remains to be evaluated. Overexpansion of orbital tissue is the key feature of TAO. In this study, the methylation profile of orbital adipose/connective tissue from TAO patients and normal individuals were compared. After screening 3,739 differentially methylated probes, the distribution and properties of these probes were analyzed. Furthermore, enriched biological functions of these genes associated with differential methylation and the relationship between their methylation status and expression profile were also identified, including PTPRU and VCAM-1. According to our results, methylation was involved in disregulated immune response and inflammation in TAO and might contribute to activation of fibroblast and adipogenesis, leading to the expansion of orbital tissue. Neuropathy and neurobehavioral symptoms were also potentially associated with methylation. These results may help to extend the understanding of methylation in TAO and provide more insights into diagnosis and treatment of patients.
Keywords
- thyroid-associated ophthalmopathy
- methylation
- orbital adipose/connective tissue
- methylation array
- pathology
