Effect of Ferrum and Manganese compounds on the glutathione content in cells of sulfurreducing bacteria Desulfuromonas acetoxidans

Abstract

Desulfuromonas acetoxidans are uncoloured gram-negative obligatory anaerobic sulfurreducing bacteria that are considered to be used as microbial-anode fuel cells with high electron recovery (>80%) from acetate oxidation to electric current as a result of their transfer in the processes of sulfur and some 3d3 type transition metals reduction, such as Ferrum and Manganese. Glutathione (GSH: l-γ-glutamil-l-cysteinilglicyn) plays the main role in redox buffer and antioxidant. This has a great sense in supporting tiol-disulfide equilibruim in the proteins and also at defense of cells against oxidative stress and the influence of different toxic compounds. The dependence between glutathione content in D. acetoxidans cells and different concentrations of ferrous (ІІ) sulfate, ferrous (III) chloride hexahydrate, manganese (II) chloride tetrahydrate and manganese (IV) oxide in growth medium has been investigated. Maximal concentration of this tripeptide in cells of investigated bacteria has been observed under the influence of 2.5 мМ MnCl2×4H2O and 0.5 mM FeSO4 on the second day of growth and under the influence of 1.0 and 0.5 mM MnO2and FeCl3×6H2O, respectively, on the third day of bacteria growth.

Keywords

• glutathione
• sulfurbacteria
• desulfuromonas acetoxidans
• ferrum
• manganese