A conformational switch in initiation factor 2 controls the fidelity of translation initiation in bacteria

Kelvin Caban; Michael Pavlov; Måns Ehrenberg; Ruben L. Gonzalez

doi:10.1038/s41467-017-01492-6

Nature Communications (Nov 2017)

A conformational switch in initiation factor 2 controls the fidelity of translation initiation in bacteria

Kelvin Caban,
Michael Pavlov,
Måns Ehrenberg,
Ruben L. Gonzalez

Affiliations

Kelvin Caban: Department of Chemistry, Columbia University
Michael Pavlov: Department of Cell and Molecular Biology, BMC, Uppsala University
Måns Ehrenberg: Department of Cell and Molecular Biology, BMC, Uppsala University
Ruben L. Gonzalez: Department of Chemistry, Columbia University

DOI: https://doi.org/10.1038/s41467-017-01492-6
Journal volume & issue: Vol. 8, no. 1
pp. 1 – 11

Abstract

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The GTP-bound form of initiation factor 2 (IF2) promotes translation initiation by accelerating 50S ribosomal subunit joining the 30S ribosomal initiation complex (30S IC). Here the authors use single-molecule FRET and ensemble rapid kinetic methods to uncover the mechanism behind IF2-mediated subunit joining.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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