The δ subunit and NTPase HelD institute a two-pronged mechanism for RNA polymerase recycling

Hao-Hong Pei; Tarek Hilal; Zhuo A. Chen; Yong-Heng Huang; Yuan Gao; Nelly Said; Bernhard Loll; Juri Rappsilber; Georgiy A. Belogurov; Irina Artsimovitch; Markus C. Wahl

doi:10.1038/s41467-020-20159-3

Nature Communications (Dec 2020)

The δ subunit and NTPase HelD institute a two-pronged mechanism for RNA polymerase recycling

Hao-Hong Pei,
Tarek Hilal,
Zhuo A. Chen,
Yong-Heng Huang,
Yuan Gao,
Nelly Said,
Bernhard Loll,
Juri Rappsilber,
Georgiy A. Belogurov,
Irina Artsimovitch,
Markus C. Wahl

Affiliations

Hao-Hong Pei: Laboratory of Structural Biochemistry, Institute of Chemistry and Biochemistry, Freie Universität Berlin
Tarek Hilal: Institute of Chemistry and Biochemistry, Research Center of Electron Microscopy and Core Facility BioSupraMol, Freie Universität Berlin
Zhuo A. Chen: Bioanalytics, Institute of Biotechnology, Technische Universität Berlin
Yong-Heng Huang: Laboratory of Structural Biochemistry, Institute of Chemistry and Biochemistry, Freie Universität Berlin
Yuan Gao: Laboratory of Structural Biochemistry, Institute of Chemistry and Biochemistry, Freie Universität Berlin
Nelly Said: Laboratory of Structural Biochemistry, Institute of Chemistry and Biochemistry, Freie Universität Berlin
Bernhard Loll: Laboratory of Structural Biochemistry, Institute of Chemistry and Biochemistry, Freie Universität Berlin
Juri Rappsilber: Bioanalytics, Institute of Biotechnology, Technische Universität Berlin
Georgiy A. Belogurov: Department of Biochemistry, University of Turku
Irina Artsimovitch: Department of Microbiology and Center for RNA Biology, The Ohio State University
Markus C. Wahl: Laboratory of Structural Biochemistry, Institute of Chemistry and Biochemistry, Freie Universität Berlin

DOI: https://doi.org/10.1038/s41467-020-20159-3
Journal volume & issue: Vol. 11, no. 1
pp. 1 – 14

Abstract

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The bacterial helicase-like transcription factor HelD interacts with the RNA polymerase (RNAP) and together with the RNAP δ subunit enhances RNAP cycling. Here, the authors present the cryo-EM structures of the monomeric and dimeric Bacillus subtilis RNAP-δ-HelD complexes and suggest a model for HelD/δ-mediated RNAP recycling and putative hibernation.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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