MOLECULAR DETECTION OF SOME VIRULENCE GENE IN Proteus Mirabilis ISOLATED FROM URINARY TRACT INFECTION IN IRAQ

Abstract

This study was concentrated for isolation and identification of 60 (35.2%) Proteus mirabilis isolates out of 170 urine samples from patients suffering from urinary tract infection from different hospitals in Baghdad city during a period from September 2020 to January 2021. The isolates were cultivated on selective media and biochemical reactions were used to identify them confirmatory APi 20 E tests. The sixty selected isolates were tested for resistance against four antibiotics. The results shown that there were differences in the antibiotic resistance of isolates. High resistance to nalidixic acid and ampicillin were found among isolates as (75%) and (51%) respectively while the resistance of Proteus mirabilis isolates to amikacin and impenem, were(8.3%). Some important virulence factor to Proteus mirabilis was detected by using molecular techniques include PCR and it was found that only 18 (60%) of isolates gave positive result for rsbA at 467 bp. 27 (90%) of them gave positive result for luxS at 464 bp.

Keywords

• Api20E, PCR, the gene s(rsbA and luxS), sensitivity test.