Applications in Plant Sciences (Mar 2013)

Development and Characterization of Microsatellite Markers in <i>Prunus sibirica</i> (Rosaceae)

  • Hua-Bo Liu,
  • Jun Liu,
  • Zhe Wang,
  • Li-Ying Ma,
  • Si-Qi Wang,
  • Xing-Gu Lin,
  • Rong-Ling Wu,
  • Xiao-Ming Pang

DOI
https://doi.org/10.3732/apps.1200074
Journal volume & issue
Vol. 1, no. 3
p. 1200074

Abstract

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Premise of the study: Microsatellite loci were developed for Prunus sibirica to investigate genetic diversity, population genetic structure, and marker-assisted selection of late-blooming cultivars in the breeding of P. sibirica. Methods and Results: Using a magnetic bead enrichment strategy, 19 primer pairs were developed and characterized across 40 individuals from three P. sibirica wild populations and six individuals of P. armeniaca. The number of alleles per locus varied from three to 11 and the observed and expected heterozygosities ranged from 0.063 to 0.917 and 0.295 to 0.876, respectively, in the three P. sibirica wild populations. All primer pairs could be successfully amplified in six individuals of P. armeniaca. Conclusions: These microsatellite primer pairs should be useful for population genetics, germplasm identification, and marker-assisted selection in the breeding of P. sibirica and related species.

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