Influence of in vitro maturation system of swine oocytes on embryo obtaining thru ICSI tecnique

Abstract

An experiment was conducted to evaluate the effects of Folligon and Corulon (hormons) supplemention during IVM on porcine oocyte maturation and subsequent fertilization with intracytoplasmic sperm injection , clevage and embryo development. Cumulus-oocyte complexes were cultured 44 hours with 10 IU Folligon and 10 IU Chorulon (0-44 h), or 22 hours withe the same amount of hormons (0-22 h) followed by a 22 hours cultue period without hormons). Nuclear maturation was assesed after IVM culture by examining the morphology and the presence of the first polar body using the micromanipulation system. The oocytes were injected with a single sperm cell and cultured in NCSU-23 medium. The percentage of cleaved embryos were determined, also the embryo development. The presence of Folligon and Chorulon during the first 22 hours of IVM gave beter results in the terms of polar body extrusion (63%) compared to the group exposed for 44 hours to the hormons (37%). After 6 days of culture , the number of advanced embyo development stage (more than 16 cells per embryo) was also significantly different in the 0-22 h group.

Keywords

• embryo
• icsi
• maturation
• oocyte
• porcine