Response to Static Magnetic Field-Induced Stress in <i>Scenedesmus obliquus</i> and <i>Nannochloropsis gaditana</i>
Génesis Serrano,
Carol Miranda-Ostojic,
Pablo Ferrada,
Cristian Wulff-Zotelle,
Alejandro Maureira,
Edward Fuentealba,
Karem Gallardo,
Manuel Zapata,
Mariella Rivas
Affiliations
Génesis Serrano
Laboratorio de Biotecnología Ambiental Aplicada, Departamento de Biotecnología, Universidad de Antofagasta, Av. Angamos 601, Antofagasta 1270300, Chile
Carol Miranda-Ostojic
Laboratorio de Biotecnología Ambiental Aplicada, Departamento de Biotecnología, Universidad de Antofagasta, Av. Angamos 601, Antofagasta 1270300, Chile
Pablo Ferrada
Centro de Desarrollo Energético Antofagasta, Universidad de Antofagasta, Av. Angamos 601, Antofagasta 1270300, Chile
Cristian Wulff-Zotelle
Laboratorio de Biología Celular, Molecular y Genética, Departamento Biomédico, Universidad de Antofagasta, Av. Angamos 601, Antofagasta 1270300, Chile
Alejandro Maureira
Laboratorio de Biotecnología Ambiental Aplicada, Departamento de Biotecnología, Universidad de Antofagasta, Av. Angamos 601, Antofagasta 1270300, Chile
Edward Fuentealba
Centro de Desarrollo Energético Antofagasta, Universidad de Antofagasta, Av. Angamos 601, Antofagasta 1270300, Chile
Karem Gallardo
Centro de Investigación Tecnológica de Agua en el Desierto (CEITSAZA), Universidad Católica del Norte, Av. Angamos 0610, Antofagasta 1270709, Chile
Manuel Zapata
Laboratorio de Biotecnología Ambiental Aplicada, Departamento de Biotecnología, Universidad de Antofagasta, Av. Angamos 601, Antofagasta 1270300, Chile
Mariella Rivas
Laboratorio de Biotecnología Ambiental Aplicada, Departamento de Biotecnología, Universidad de Antofagasta, Av. Angamos 601, Antofagasta 1270300, Chile
Magnetic fields in biological systems is a promising research field; however, their application for microalgae has not been fully exploited. This work aims to measure the enzymatic activity and non-enzymatic activity of two microalgae species in terms of superoxide dismutase (SOD), catalase (CAT), and carotenoids, respectively, in response to static magnetic fields-induced stress. Two magnet configurations (north and south) and two exposure modes (continuous and pulse) were applied. Two microalgae species were considered, the Scenedesmus obliquus and Nannochloropsis gaditana. The SOD activity increased by up to 60% in S. obliquus under continuous exposure. This trend was also found for CAT in the continuous mode. Conversely, under the pulse mode, its response was hampered as the SOD and CAT were reduced. For N. gaditana, SOD increased by up to 62% with the south configuration under continuous exposure. In terms of CAT, there was a higher activity of up to 19%. Under the pulsed exposure, SOD activity was up to 115%. The CAT in this microalga was increased by up to 29%. For N. gaditana, a significant increase of over 40% in violaxanthin production was obtained compared to the control, when the microalgae were exposed to SMF as a pulse. Depending on the exposure mode and species, this methodology can be used to produce oxidative stress and obtain an inhibitory or enhanced response in addition to the significant increase in the production of antioxidant pigments.