Molecular Mechanisms of Breast Cancer Drug Resistance and CRISPR/Cas9 Strategies to Overcome

Abstract

The most frequent cancer among women and a significant contributor to mortality is breast cancer. The CRISPR/Cas9 gene-editing tool has promising applications for BC drug resistance, which is a unique and creative approach that has lately attracted attention and can be used to fix multidrug resistance-related gene alterations. Recent research has effectively investigated and targeted particular genes linked to BC treatment resistance using CRISPR/Cas9 gene editing, including those linked to hormone receptor signaling, drug efflux transporters, and DNA repair pathways. The CRISPR/Cas9 technology's selective disruption or mutation of these genes provides valuable information about their role in resistance and paves the path for cutting-edge treatment options. Despite the advantages, there are limitations in the study on CRISPR/Cas9-based gene editing for BC treatment resistance, for example, off-target effects and the improvement of delivery techniques are still major issues. Successful clinical translation depends on methods to improve the specificity and effectiveness of CRISPR/Cas9 editing and to solve these constraints. CRISPR/Cas9 gene editing has the ability to overcome BC treatment resistance by identifying crucial genetic variables and revealing new therapeutic targets. This review aims to explore the possibility of CRISPR/Cas9 gene editing as a cutting-edge method of combating BC medication resistance.

Keywords

• breast cancer
• drug resistance
• crispr/cas9