CHARACTERISTICS OF NEUTROPHIL CHEMILUMINESCENCE IN PATIENTS WITH TYPE G MULTIPLE MYELOMA AT DIFFERENT STAGES OF THE DISEASE

Abstract

The aim of present work was to study some activation features of neutrophilic granulocytes from peripheral blood of the patients with G type of multiple myeloma (MM), as depending on clinical stage of the disorder. In this study, a number of parameters were evaluated, as follows: time dynamics of the up-to-maximum luminescence increase; maximal luminescence levels, areas under the curves (AUC) for the dynamic luminescence registration. Zymosan was used as chemiluminescence inducer, and luminol, as enhancer of the “respiratory burst”. The chemiluminescence enhancement was assessed as a ratio of AUC values corresponding to induced-to-spontaneous chemiluminescence being denoted as an ‘activation index’. The onset and progression of MM in humans was accompanied by disturbance of neutrophil functions. MM patients at stage II and III exhibited a significant increase of spontaneous ROS production relative to controls. The inducible ROS production by neutrophils was also increased in all groups, as compared with spontaneous production and and control parameters, thus arguing for a distinct role of neutrophils, as potentially cytotoxic effectors against tumor cells, along with their basic function of nonspecific antimicrobial protection.

Keywords

• multiple myeloma
• neutrophilic granulocytes
• chemiluminescence