<i>Lactobacillus delbrueckii</i> Protected Intestinal Integrity, Alleviated Intestinal Oxidative Damage, and Activated Toll-Like Receptor–Bruton’s Tyrosine Kinase–Nuclear Factor Erythroid 2-Related Factor 2 Pathway in Weaned Piglets Challenged with Lipopolysaccharide

Fengming Chen; Jiayi Chen; Qinghua Chen; Lingyuan Yang; Jie Yin; Yinghui Li; Xingguo Huang

doi:10.3390/antiox10030468

Antioxidants (Mar 2021)

<i>Lactobacillus delbrueckii</i> Protected Intestinal Integrity, Alleviated Intestinal Oxidative Damage, and Activated Toll-Like Receptor–Bruton’s Tyrosine Kinase–Nuclear Factor Erythroid 2-Related Factor 2 Pathway in Weaned Piglets Challenged with Lipopolysaccharide

Fengming Chen,
Jiayi Chen,
Qinghua Chen,
Lingyuan Yang,
Jie Yin,
Yinghui Li,
Xingguo Huang

Affiliations

Fengming Chen: College of Animal Science and Technology, Hunan Agricultural University, Changsha 410128, China
Jiayi Chen: College of Animal Science and Technology, Hunan Agricultural University, Changsha 410128, China
Qinghua Chen: College of Animal Science and Technology, Hunan Agricultural University, Changsha 410128, China
Lingyuan Yang: College of Animal Science and Technology, Hunan Agricultural University, Changsha 410128, China
Jie Yin: College of Animal Science and Technology, Hunan Agricultural University, Changsha 410128, China
Yinghui Li: College of Animal Science and Technology, Hunan Agricultural University, Changsha 410128, China
Xingguo Huang: College of Animal Science and Technology, Hunan Agricultural University, Changsha 410128, China

DOI: https://doi.org/10.3390/antiox10030468
Journal volume & issue: Vol. 10, no. 3
p. 468

Abstract

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Oxidative stress is increasingly being recognized as a player in the pathogenesis of intestinal pathologies, and probiotics are becoming an attractive means of addressing it. The present study investigated the effects of dietary supplementation with Lactobacillus delbrueckii (LAB) on intestinal integrity and oxidative damage in lipopolysaccharide (LPS)-challenged piglets. A total of 36 crossbred weaned piglets (Duroc × Landrace × Large Yorkshire) were randomly divided into three groups: (1) non-challenged controls (CON), (2) LPS-challenged controls (LPS), and (3) 0.2% LAB (2.01 × 1010 CFU/g) + LPS treatment (LAB + LPS). On the 29th day of the experiment, the LPS and CON groups were injected intraperitoneally with LPS and saline at 100 ug/kg body weight, respectively. The results show that the LPS-induced elevation of the serum diamine oxidase (DAO) level and small intestinal crypt depth (CD) were reversed by the dietary addition of LAB, which also markedly increased the ileal expression of tight junction proteins (occludin, ZO-1, and claudin-1) in the LPS-challenged piglets. Furthermore, LAB supplementation normalized other LPS-induced changes, such as by decreasing malondialdehyde (MDA) in both the serum and intestinal mucosa and 8-hydroxy-2-deoxyguanosine (8-OHdG) in the jejunal mucosa, increasing glutathione reductase (GR) and glutathione peroxidase (GSH-Px) in both the serum and intestinal mucosa, and increasing glutathione (GSH) and superoxide dismutase (SOD) in the jejunal mucosa. LAB also activated Toll-like receptor (TLR)–Bruton’s tyrosine kinase (Btk)–nuclear factor erythroid 2-related factor 2(Nrf2) signaling pathways in the intestine, suggesting that it plays a vital role in the ameliorative antioxidant capacity of weaned piglets. In summary, LAB increased intestinal integrity by improving the intestinal structure and tight junctions while enhancing antioxidant functions via the activation of the TLR–Btk–Nrf2 signaling pathway.

Published in Antioxidants

ISSN: 2076-3921 (Online)
Publisher: MDPI AG
Country of publisher: Switzerland
LCC subjects: Medicine: Therapeutics. Pharmacology
Website: http://www.mdpi.com/journal/antioxidants

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