RSV glycoprotein and genomic RNA dynamics reveal filament assembly prior to the plasma membrane

Daryll Vanover; Daisy V. Smith; Emmeline L. Blanchard; Eric Alonas; Jonathan L. Kirschman; Aaron W. Lifland; Chiara Zurla; Philip J. Santangelo

doi:10.1038/s41467-017-00732-z

Nature Communications (Sep 2017)

RSV glycoprotein and genomic RNA dynamics reveal filament assembly prior to the plasma membrane

Daryll Vanover,
Daisy V. Smith,
Emmeline L. Blanchard,
Eric Alonas,
Jonathan L. Kirschman,
Aaron W. Lifland,
Chiara Zurla,
Philip J. Santangelo

Affiliations

Daryll Vanover: Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University
Daisy V. Smith: Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University
Emmeline L. Blanchard: Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University
Eric Alonas: Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University
Jonathan L. Kirschman: Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University
Aaron W. Lifland: Institute of Bioengineering and Bioscience, Georgia Institute of Technology
Chiara Zurla: Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University
Philip J. Santangelo: Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University

DOI: https://doi.org/10.1038/s41467-017-00732-z
Journal volume & issue: Vol. 8, no. 1
pp. 1 – 15

Abstract

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Assembly of filamentous RSV particles is incompletely understood due to a lack of techniques suitable for live-cell imaging. Here Vanover et al. use labeled soybean agglutinin to selectively label RSV G protein and show how filamentous RSV assembly, initiated in the cytoplasm, uses G protein recycled from the plasma membrane.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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