Physicochemical, microbiological characterization and phytotoxicity of digestates produced on single-stage and two-stage anaerobic digestion of food waste

Abstract

Abstract Organic amendments favor the development of sustainable agriculture by using less chemical fertilizers. In this way, the use of digestates from anaerobic digestion as soil conditioners in agriculture has been gaining interest due to their important N and P nutrient contents, among others. This study evaluated the potential use of digestates from anaerobic reactors treating food waste in single (D1) and two-stages (D2: hydrolytic/acidogenic and D3: acetogenic/methanogenic) configurations. Digestate characteristics and their potential application conditions (100, 50, 25, and 5%) were evaluated using Raphanus sativus as an indicator species. D3 reported the best performance in terms of: (i) better physicochemical, microbiological, and parasitological characteristics, being a class B material, without exceeding the established limits for heavy metals, fecal coliforms (FC 0.87 and acidic pH); and (iii) greater potential for agricultural use, since a 5% dose produced a germination index > 120%, whose effect is associated with the presence of humic and fulvic acids and with N and P concentrations > 1%. In addition, the study reported that volatile fatty acids > 2500 mg L− 1 act as antimicrobial agents, reducing the required pathogen removal pretreatments.

Keywords

• Digestate
• Food waste
• Germination
• Organic fertilizers
• Single and two-stage anaerobic digestion
• Sustainable agriculture