Callose deposited at soybean sieve element inhibits long-distance transport of Soybean mosaic virus
Jie Zhang,
Na Liu,
Aihua Yan,
Tianjie Sun,
Xizhe Sun,
Guibin Yao,
Dongqiang Xiao,
Wenlong Li,
Chunyan Hou,
Chunyan Yang,
Dongmei Wang
Affiliations
Jie Zhang
State Key Laboratory of North China Crop Improvement and Regulation/ Hebei Key Laboratory of Plant Physiology and Molecular Pathology/College of Life Sciences, Hebei Agricultural University
Na Liu
State Key Laboratory of North China Crop Improvement and Regulation/ Hebei Key Laboratory of Plant Physiology and Molecular Pathology/College of Life Sciences, Hebei Agricultural University
Aihua Yan
State Key Laboratory of North China Crop Improvement and Regulation/ Hebei Key Laboratory of Plant Physiology and Molecular Pathology/College of Life Sciences, Hebei Agricultural University
Tianjie Sun
State Key Laboratory of North China Crop Improvement and Regulation/ Hebei Key Laboratory of Plant Physiology and Molecular Pathology/College of Life Sciences, Hebei Agricultural University
Xizhe Sun
State Key Laboratory of North China Crop Improvement and Regulation/ Hebei Key Laboratory of Plant Physiology and Molecular Pathology/College of Life Sciences, Hebei Agricultural University
Guibin Yao
State Key Laboratory of North China Crop Improvement and Regulation/ Hebei Key Laboratory of Plant Physiology and Molecular Pathology/College of Life Sciences, Hebei Agricultural University
Dongqiang Xiao
State Key Laboratory of North China Crop Improvement and Regulation/ Hebei Key Laboratory of Plant Physiology and Molecular Pathology/College of Life Sciences, Hebei Agricultural University
Wenlong Li
State Key Laboratory of North China Crop Improvement and Regulation/ Hebei Key Laboratory of Plant Physiology and Molecular Pathology/College of Life Sciences, Hebei Agricultural University
Chunyan Hou
State Key Laboratory of North China Crop Improvement and Regulation/ Hebei Key Laboratory of Plant Physiology and Molecular Pathology/College of Life Sciences, Hebei Agricultural University
Chunyan Yang
Institute of Cereal and Oil Crops, Hebei Academy of Agriculture and Forestry Sciences
Dongmei Wang
State Key Laboratory of North China Crop Improvement and Regulation/ Hebei Key Laboratory of Plant Physiology and Molecular Pathology/College of Life Sciences, Hebei Agricultural University
Abstract The function of callose and its deposition characteristics at phloem in the resistance to the long-distance transportation of Soybean mosaic virus (SMV) through phloem was studied. Two different methods of SMV inoculation were used in the study, one was direct friction of the virus on seedling leaves and the other was based on grafting scion and rootstock to create different resistance and sensitivity combinations. Veins, petioles of inoculated leaves and rootstock stems were stained with callose specific dye. Results from fluorescence microscope observation, pharmacological test, and PCR detection of SMV coat protein gene (SMV-CP) showed the role of callose in long-distance transportation of SMV through phloem during infection of soybean seedlings. When the inhibitor of callose synthesis 2-deoxy-D-glucose (2-DDG) was used, the accumulation of callose fluorescence could hardly be detected in the resistant rootstocks. These results indicate that callose deposition in phloem restricts the long-distance transport of SMV, and that the accumulation of callose in phloem is a main contributing factor for resistance to this virus in soybean.
